| 引用本文:李伟1，李春根2，穆晓红3，陈黎明4，陈琛1，赵子义3.基于网络药理学预测金匮肾气丸治疗骨质疏松症的作用机制[J].世界中医药,2021,(22):. |
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| 基于网络药理学预测金匮肾气丸治疗骨质疏松症的作用机制 |
| Mechanism of Jinkui Shenqi Pill inTreatment of Osteoporosis Based on Network Pharmacology |
| 投稿时间：2020-11-13 |
| DOI：10.3969/j.issn.1673-7202.2021.22.004 |
| 中文关键词: 金匮肾气丸 骨质疏松症 网络药理学 药物成分 作用靶点 信号通路 作用机制 |
| English Keywords:Jinkui Shenqi Pill Osteoporosis Network pharmacology Drug components Target of action Signaling pathway Mechanism of action |
| 基金项目:国家自然科学基金面上项目（81874467） |
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| 中文摘要: |
| 目的：基于网络药理学预测金匮肾气丸治疗骨质疏松症的作用机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）、中药分子机制综合数据库（TCMID）、中药分子机制生物信息学分析工具（BATMAN-TCM）等数据库检索金匮肾气丸药物成分及成分相关靶点；以“Osteoporosis”为关键词在人类基因数据库（GeneCards）、在线人类孟德尔遗传数据库（OMIM）、DrugBank等数据库检索骨质疏松症疾病相关靶点；制作韦恩图，获取药物-疾病交集靶点；利用String数据库、Cytoscape 3.6.1软件构建药物-疾病交集靶点蛋白互作网络及药物-成分-疾病-靶点网络；通过DAVID数据库对药物-疾病交集靶点进行基因本体（GO）、京都基因和基因组百科全书（KEGG）富集分析，并将富集结果可视化。结果：共获得金匮肾气丸药物成分118个，包括槲皮素、山柰酚、大黄素等；有效成分相关靶点202个，疾病相关靶点1 856个，药物-疾病交集靶点87个，包括前列腺素内过氧化物合酶2（PTGS2）、转录因子（JUN）、胞间黏附分子1（ICAM1）等；GO富集分析功能条目376条，其中生物过程（BP）290条，细胞组分（CC）28条，分子功能（MF）58条，包括RNA聚合酶Ⅱ启动子的转录正调控、类固醇激素受体活性等；KEGG信号通路共90条，包括类风湿性关节炎信号通路、破骨细胞分化信号通路、催乳激素信号通路等。结论：金匮肾气丸通过槲皮素、山柰酚、大黄素、薯蓣皂苷元、β-谷甾醇等有效成分作用于PTGS2、JUN、ICAM1、白细胞介素-1β（IL-1β）、基质金属蛋白酶9（MMP9）等关键靶点通过抗氧化、抑制炎症反应、促进成骨细胞增殖分化、抑制破骨细胞骨吸收等作用机制达到治疗OP的作用。 |
| English Summary: |
| To predict the mechanism of Jinkui Shenqi Pill in the treatment of osteoporosis based on network pharmacology.Methods TCMSP,TCMID，BATMAN-TCM databases were used to retrieve the drug components and related targets of Jinkui Shenqi pill; the related targets of osteoporosis were searched in GeneCards，OMIM，DrugBank databases with the key word of “osteoporosis”; the common target of the drug-disease were obtained by generating Venny diagrams; string database and Cytoscape 3.6.1 software were used to construct the drug-disease common target protein interaction network and drug-component-disease-target network; finally，GO and KEGG enrichment analysis of common target were carried out through David database，and the enrichment results were visualized.Result A total of 118 chemical components from Jinkui Shenqi pill were obtained，including quercetin，kaempferol，emodin，etc.; there were 202 active component related targets，1856 disease-related targets and 87 drug-disease common targets，including PTGS2，JUN，ICAM1，etc.; there were 376 functional items analyzed by GO enrichment analysis，namely 290 biological processes (BP)，28 cellular components (CC) and 58 molecular functions (MF)，including the positive regulation of transcription from RNA polymerase Ⅱ promoter and steroid hormone receptor activity; there were 90 KEGG signaling pathways，mainly including rheumatoid arthritis signaling pathway，osteoclast differentiation signaling pathway，prolactin signaling pathway，etc.Conclusion Jinkui Shenqi Pill functions mainly through quercetin，kaempferol，emodin，diosgenin and beta-sitosterol on key target genes of PTGS2，JUN，ICAM1，IL1B and MMP9 through the mechanism of anti-oxidation，inhibition of inflammatory reaction，promotion of osteoblast proliferation and differentiation，inhibition of osteoclast bone absorption，and finally achieve the therapeutic effect of treat OP. |
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