| 引用本文:韩甜甜1，李丹华2，刘小虹2，詹少峰2，江勇3.基于网络药理学的血必净治疗H1N1病毒引起的肺微血管渗漏的潜在机制[J].世界中医药,2021,(22):. |
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| 基于网络药理学的血必净治疗H1N1病毒引起的肺微血管渗漏的潜在机制 |
| Potential Mechanism of Xuebijing in Treatment of H1N1 Virus-Induced Pulmonary Microvascular Leakage Based on Network Pharmacology |
| 投稿时间：2020-11-19 |
| DOI：10.3969/j.issn.1673-7202.2021.22.006 |
| 中文关键词: 血必净注射液 H1N1病毒 肺微血管渗漏 内皮细胞 潜在机制 内皮屏障 网络药理学 病毒性肺炎 |
| English Keywords:Xuebijing injection H1N1 Pulmonary microvascular leakage Endothelial cells Potential mechanism Endothelial barrier Network pharmacology Viral pneumonia |
| 基金项目:国家自然科学基金项目（81973814）；广东省重点领域研发计划（第五批）项目（粤财科教【2020】52号） |
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| 中文摘要: |
| 目的:探讨血必净注射液干预肺微血管渗漏辅助治疗H1N1病毒性肺炎的潜在药理作用机制。方法:收集血必净注射液中的活性成分，反向预测潜在作用靶点。从GeneCards数据库中获得疾病相关靶点。使用Venny工具对药物及疾病的潜在靶点进行交集分析，并对交集靶点进行蛋白质-蛋白质相互作用（PPI）网络的构建。利用DAVID和京都基因和基因组百科全书（KEGG）数据库进行通路注释，采用Cytoscape软件构建“中药-活性成分-靶点-疾病”网络模型。结果：共筛选出25个活性分子，通过调控TP53、VEGFA、EGFR、TNF、TLR4、CASP3、JUN等关键基因，影响核因子κB信号通路、HIF-1信号通路、T细胞受体信号通路、细胞凋亡信号转导途径、血管内皮生长因子信号通路和Toll样受体信号转导途径等91条通路发挥抗炎、调节免疫等作用。结论：本研究明确了血必净注射液抗炎和调节免疫作用的物质基础,为进一步阐释血必净注射液干预肺微血管渗漏辅助治疗H1N1病毒性肺炎相关机制提供科学依据。 |
| English Summary: |
| To explore the potential pharmacological mechanism of Xuebijing injection in the intervention of pulmonary microvascular leakage in the adjuvant treatment of H1N1 viral pneumonia.Methods:Active ingredients in Xuebijing injection were collected，and the potential targets were prediected.Disease-related targets were obtained from the Genecards database.The Venny tool was used to analyze the intersection of potential targets of drugs and diseases，and a PPI network for the intersection targets was constructed.DAVID and KEGG databases were retrieved for pathway annotation,using Cytoscape software to construct a network model of “Chinese medicine-active ingredients-targets-disease”.Results:A total of 25 active molecules were screened out，which can regulate key genes of TP53、VEGFA、EGFR、TNF、TLR4、CASP3 and JUN to affect 91 pathways，including the NF-κB signaling pathway，HIF-1 signaling pathway，and TTR，apoptosis signaling pathway，vascular endothelial growth factor signaling pathway，and Toll-like receptor signaling pathway，with anti-inflammatory and immune regulation functions.Conclusion:This study clarifies the material basis of Xuebijing injection's anti-inflammatory and immune regulation effects，and provides a scientific basis for further elucidating the mechanism of Xuebijing injection's intervention in pulmonary microvascular leakage adjuvant treatment of H1N1 viral pneumonia. |
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