| 引用本文:柯来顺1，涂燕芬2，林庆金1，刘华斌1，肖雪莲1.牛蒡子苷元对阿尔茨海默病体外模型细胞凋亡和氧化损伤的影响[J].世界中医药,2021,(22):. |
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| 牛蒡子苷元对阿尔茨海默病体外模型细胞凋亡和氧化损伤的影响 |
| Arctigenin Inhibits Cell Apoptosis and Oxidative Damage in an in Vitro Model of Alzheimer's Disease |
| 投稿时间：2021-09-01 |
| DOI：10.3969/j.issn.1673-7202.2021.22.010 |
| 中文关键词: 牛蒡子苷元 PC12细胞 阿尔茨海默病 β淀粉样肽25-35 AKT信号 凋亡 氧化损伤 增殖 |
| English Keywords:Arctigenin PC12 cells Alzheimer's disease Aβ25-35 AKT signal Apoptosis Oxidative damage Proliferation |
| 基金项目:国家科技支撑计划项目(2009BA177B08) |
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| 中文摘要: |
| 目的:探讨牛蒡子苷元对阿尔茨海默病体外模型β淀粉样肽25-35(Aβ25-35)诱导PC12细胞凋亡和氧化损伤的影响及机制。方法:将PC12细胞分成对照组、模型组(Aβ25-35处理)、ATG-L组(2 μmol/L牛蒡子苷元和Aβ25-35处理)、ATG-M组(4 μmol/L牛蒡子苷元和Aβ25-35处理)、ATG-H组(8 μmol/L牛蒡子苷元和Aβ25-35处理)、ATG-H+LY29400组(AKT信号抑制剂LY29400、8 μmol/L牛蒡子苷元和Aβ25-35处理)。以流式细胞术检测细胞凋亡变化,黄嘌呤氧化法检测超氧化物歧化酶(SOD)含量,Western Blotting法检测磷酸化的蛋白激酶B(p-AKT)蛋白表达。结果:ATG-L组、ATG-M组、ATG-H组p-AKT/AKT表达量以及SOD含量高于模型组,差异均有统计学意义(均P<0.05);ATG-L组、ATG-M组、ATG-H组细胞凋亡率低于模型组,差异均有统计学意义(均P<0.05);ATG-H+LY29400组p-AKT/AKT表达量以及SOD含量低于ATG-H组,差异均有统计学意义(均P<0.05);ATG-H+LY29400组细胞凋亡率高于ATG-H组,差异均有统计学意义(均P<0.05)。结论:牛蒡子苷元通过激活AKT信号通路抑制阿尔茨海默病体外模型Aβ25-35诱导PC12细胞凋亡和氧化损伤。 |
| English Summary: |
| To investigate the effect and its mechanis of arctigenin on the apoptosis and oxidative damage of PC12 cells induced by amyloid beta 25-35(Aβ25-35)in an in vitro model of Alzheimer's disease.Methods:PC12 cells were divided into a control group，a model group (Aβ25-35 treatment)，a ATG-L group (2 μmol/L arctigenin and Aβ25-35 treatment)，a ATG-M group (4 μmol/L arctigenin and Aβ25-35 treatment)，a ATG-H group (8 μmol/L arctigenin and Aβ25-35 treatment)，and a ATG-H+LY29400 group (AKT signal inhibitor LY29400,8 μmol/L arctigenin and Aβ25-35 treatment).Flow cytometry was used to detect cell apoptosis changes.Xanthine oxidation method was used to detect superoxide dismutase (SOD) content.The expression of Phosphorylated protein kinase B (p-AKT) protein in cells was detected with Western Blotting method.Results:P-AKT/AKT expression and SOD content in the ATG-L,ATG-M,ATG-H group were higher than those in the Model group，and the differences were statistically significant (Ps＜0.05); The apoptosis rate of the ATG-L,ATG-M,ATG-H group were lower than those of the Model group，and the difference was statistically significant (Ps＜0.05); The p-AKT/AKT,SOD levels in the ATG-H+LY29400 group were lower than those in the ATG-L，ATG-M and ATG-H group，and the differences were statistically significant (Ps＜0.05); The apoptosis rate in the ATG-H+LY29400 group were higher than those in the ATG-H group，and the differences were statistically significant (Ps＜0.05).Conclusion:Arctigenin can inhibit the apoptosis and oxidative damage of PC12 cells induced by Aβ25-35 in an in vitro model of Alzheimer's disease by activating the AKT signaling pathway. |
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