| 引用本文:周怡驰1，晏军1，李玲1，冉云2，胡世平2，党志博1，苏晓鹏1.基于肝气虚证与网络药理学探讨黄芪-柴胡干预肝炎的作用机制[J].世界中医药,2022,(04):. |
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| 基于肝气虚证与网络药理学探讨黄芪-柴胡干预肝炎的作用机制 |
| Mechanisms of Astragalus-radix Bupleuri in Intervening Hepatitis Based on Liver Qi Deficiency Syndrome and Network Pharmacology |
| 投稿时间：2021-01-26 |
| DOI：10.3969/j.issn.1673-7202.2022.04.001 |
| 中文关键词: 黄芪 柴胡 肝气虚证 肝炎 网络药理 分子对接 槲皮素 氧化应激 |
| English Keywords:Astragalus Radix bupleuri Syndrome of liver qi deficiency Hepatitis Network pharmacology Molecular docking Quercetin Oxidative stress |
| 基金项目:国家自然科学基金项目(81973733) |
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| 中文摘要: |
| 目的:从肝气虚证角度并通过网络药理学探讨黄芪-柴胡干预肝炎的作用机制。方法:检索中药系统药理学数据库与分析平台（TCMSP）等数据库,筛选黄芪-柴胡干预肝炎的活性成分和作用靶点。构建化合物-靶点-疾病网络和蛋白质-蛋白质相互作用（PPI） 网络,进行拓扑学分析,筛选关键药效分子和核心靶点。对作用靶点进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析,并用Cytoscape软件构建化合物-靶点-通路网络。结果:黄芪-柴胡通过升补肝气治疗肝炎患者肝气虚证。经网络药理学分析,筛选出黄芪-柴胡14个关键药效分子,19个核心作用靶点与其干预肝炎密切相关。分子对接结果显示,度值前10的核心靶点能与黄芪-柴胡的关键活性成分形成较稳定的构象,具有良好的结合活性。GO和KEGG结果主要涉及炎症、氧化应激、细胞增殖等生物学过程。结论:黄芪-柴胡或通过槲皮素、山柰酚、芒柄花素等活性成分作用于Toll样受体、Th17细胞分化、Th1和Th2细胞分化、PI3K/AKT、MAPK、IL-17等信号通路,参与调节氧化应激反应、肝脏炎症反应、细胞增殖等生物学过程,发挥干预肝炎的作用。 |
| English Summary: |
| Investigating the theoretical foundation of Astragalus-Radix bupleuri in treating patients with hepatitis from liver qi deficiency syndrome and exploring the possible mechanisms of Astragalus-Radix bupleuri in intervening hepatitis by using the method of network pharmacology.Methods:Effective components and targets of Astragalus-Radix bupleuri in intervening hepatitis were obtained from TCMSP and other databases.Then,“component-target-disease” network and protein-protein interaction(PPI) network were established in Cytoscape and STRING database respectively,to ascertain the key efficacy components and targets.Molecular docking simulations were evaluated through Autodock vina software.Finally,GO and KEGG enrichment analysis were performed,and “compound-target-signaling pathway” network was also established in Cytoscape.Results:Astragalus-Radix bupleuri can treat patients with hepatitis of liver qi deficiency by lifting and tonifying liver qi.The fourteen core active components and nineteen key targets of Astragalus-Radix bupleuri were closely related to the intervention of hepatitis.Molecular docking results indicated that the top 10 key targets of degree values can form stable conformations with key compounds of RB and had favorable binding activities.Go and KEGG analysis revealed that the targets of Astragalus-Radix bupleuri in intervening hepatitis significantly enriched in biological processes such as inflammation,oxidative stress,cell proliferation and TLR signaling pathway.Conclusion:Astragalus-Radix bupleuri acts on Toll-like receptors,Th17 cell differentiation,Th1 and Th2 cell differentiation,PI3K/AKT,MAPK,IL-17 and other signaling pathways through active components such as quercetin,kaempferol,and formononetin.It participates in the regulation of oxidative stress response,liver inflammatory response,cell proliferation and other biological processes,and plays a role in intervening hepatitis. |
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