| 引用本文:李慧祯1，童丽1，陈秀萍1，李永平1，孙文萍2.穴位埋线对多囊卵巢综合征胰岛素抵抗模型大鼠慢性炎症的疗效评价[J].世界中医药,2022,(04):. |
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| 穴位埋线对多囊卵巢综合征胰岛素抵抗模型大鼠慢性炎症的疗效评价 |
| Effect of Acupoint Catgut Embedding on Chronic Inflammation in Rats with Insulin Resistance Model of Polycystic Ovary Syndrome |
| 投稿时间：2021-01-25 |
| DOI：10.3969/j.issn.1673-7202.2022.04.006 |
| 中文关键词: 穴位埋线 多囊卵巢综合征 胰岛素抵抗 慢性炎症 C反应蛋白 游离雄激素指数 性激素结合球蛋白 |
| English Keywords:Acupoint catgut embedding Polycystic ovarian syndrome Insulin resistance Chronic inflammation CRP FAI SHBG |
| 基金项目:中医药高层次人才培训基地建设项目(国中医药人教函[2019]128号);青海省卫健委中藏医药研究课题(2019-wjzdx-69)——针药干预对PCOS-IR模型大鼠慢性炎症的分子机制研究 |
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| 中文摘要: |
| 目的:探讨穴位埋线对多囊卵巢综合征胰岛素抵抗(PCOS-IR)模型大鼠的疗效。方法:将48只雌性SD大鼠按随机数字表法分为正常组、模型组、二甲双胍组和穴位埋线组,每组8只。除正常组外,其余各组以项背部皮下注射脱氢表雄酮建立PCOS-IR模型。按照每组治疗方案进行干预,共28 d。借助HE染色对卵巢组织形态学变化进行观察;酶联免疫吸附试验(ELISA)法对血清空腹胰岛素(FINS)、C反应蛋白(CRP)、黄体生成素(LH)、性激素结合球蛋白(SHBG)和睾酮(T)进行定量,通过相关公式计算稳态模型胰岛素抵抗指数(HOMA-IR)和游离雄激素指数(FAI)值。结果:与正常组比较,模型组卵巢组织黄体、正常卵泡少见,闭锁卵泡数增多,闭锁卵泡内见大量的颗粒细胞核固缩、崩解,颗粒层细胞被挤压至边缘,排列无序,见大量囊样扩张卵泡,且大鼠血清FINS、FBG、HOMA-IR、CRP、LH、T及FAI水平显著升高(P<0.01),SHBG水平降低(P<0.05)。与模型组比较,各观察组卵巢中黄体量增多,囊泡及闭锁卵泡减少,颗粒层细胞规律排列,尤其联合组各级卵泡数量及发育接近正常,未见囊样改变的卵泡,且大鼠血清FINS、FBG、HOMA-IR、CRP、T、FAI、LH水平均显著降低(P<0.01),SHBG水平升高(P<0.05)。结论:穴位埋线可以改善PCOS-IR模型大鼠性激素水平、胰岛素抵抗(IR)及慢性炎症状态,该疗法方便简洁、安全性高、不良反应少,值得临床推广。 |
| English Summary: |
| To investigate the therapeutic effect of catgut embedding at acupoint on insulin resistance(PCOS-IR) in rats with ovarian syndrome.Methods:All 48 female SD rats were randomly divided into a normal group,a model group,a metformin group and an acupoint catgut embedding group,eight in each group.Except for the normal group,PCOS-IR model was established by subcutaneous injection of dehydroepiandrosterone into the rest of the groups.Interventions were according to each treatment plan,lasting for 28 days.Histomorphology changes of ovary were observed by HE staining; ELISA methods were used to quantify serum FINS,CRP,LH,SHBG and keto testosterone(T).The HOMA-IR and FAI values were calculated with relevant formulas.Results:Comparing with the normal group,ovarian corpus luteum,normal follicles were rare in the model group,with the number of atresia follicles increased and a large number of granulosa cell nuclei shrank and disintegrated in atresia follicles,granulosa cells being squeezed to the margin,arranged disorderedly; and a large number of cystic dilated follicles were found in the model group; serum FINS,FBG,HOMA-IR,CRP,LH,T and FAI levels in rats increased significantly(P<0.01) and SHBG levels decreased(P<0.05).When compared with the model group,the corpus luteum increased,vesicles and atresia follicles decreased; granulosa cells were arranged regularly,especially that the number and development of follicles at all levels in the combined group were close to normal level; and no cystic changes were found; the serum FINS,FBG,HOMA-IR,CRP,T,FAI,LH level of rats decreased significantly(P<0.01) and SHBG level increased(P<0.05).Conclusion:Acupoint catgut embedding therapy can improve the level of sex hormones,IR and chronic inflammation in PCOS-IR model rats.This therapy is convenient,simple,safe and it has less side effects.It is worthy of being widely popularized in the clinical application. |
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