| 引用本文:王顺意1，张军2，刘颖1，陈伟1，魏锦花1，王保苍1，刘文超1.益气养阴活血方改善2型糖尿病大鼠胰岛分泌功能的作用机制[J].世界中医药,2022,(04):. |
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| 益气养阴活血方改善2型糖尿病大鼠胰岛分泌功能的作用机制 |
| Mechanism of Yiqi Yangyin Huoxue Formula on Improving Islet Secretion Function in Type 2 Diabetic Rats |
| 投稿时间：2020-08-10 |
| DOI：10.3969/j.issn.1673-7202.2022.04.007 |
| 中文关键词: 益气养阴活血方 2型糖尿病 链脲佐菌素 微小核糖核酸-375 空腹血糖 胰岛分泌功能 胰岛细胞凋亡 胱天蛋白酶-3 |
| English Keywords:Yiqi Yangyin Huoxue Formula Type 2 diabetes mellitus Streptozotocin Microribonucleic acid-375 Fasting blood glucose Islet secretion function Islet cell apoptosis Cysteine-containing aspartate proteolytic enzyme-3 |
| 基金项目:河北省中医药管理局科研计划项目(2017129) |
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| 中文摘要: |
| 目的:探讨益气养阴活血方改善2型糖尿病(T2DM)大鼠胰岛分泌功能的作用机制。方法:选取无特定病原体（SPF）级雄性SD大鼠66只,随机分为对照组11只及造模组55只。造模组用高糖高脂饲料喂养联合链脲佐菌素建立T2DM模型,对照组给予标准颗粒饲料喂养及等体积0.1 mmol/L柠檬酸-柠檬酸钠缓冲液。造模成功的大鼠分为模型组、益气养阴活血方低、中、高剂量组及西药组，每组11只。模型组及对照组给予蒸馏水,益气养阴活血方低、中、高剂量组给予0.43 g/mL、0.86 g/mL、1.72 g/mL益气养阴活血方;西药组予以20 mg/mL二甲双胍悬液;各组灌胃剂量10 mL/kg,1次/d,连续12周。检测比较空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素分泌指数(HOMA-β)、胰岛细胞凋亡指数、胰岛微小核糖核酸-375(miR-375)及B淋巴细胞瘤-2(Bcl-2)、胱天蛋白酶-3(Caspase-3)表达水平。结果:与模型组比较,各组FBG水平、胰岛细胞凋亡指数、胰岛组织miR-375及Caspase-3表达水平较低,FINS、HOMA-β水平及胰岛组织Bcl-2表达水平较高,差异均有统计学意义(P<0.05),且益气养阴活血方组呈一定量效关系。结论:益气养阴活血方能剂量依赖性的降低T2DM大鼠血糖,改善胰岛分泌功能,抑制胰岛细胞凋亡,其作用机制可能与抑制胰岛组织miR-375表达,调节凋亡相关蛋白Bcl-2、Caspase-3表达有关。 |
| English Summary: |
| To explore the mechanism of Yiqi Yangyin Huoxue Formula on improving islet secretion function in rats with type 2 diabetes mellitus(T2DM).Methods:A total of 66 SPF male SD rats were selected,and were randomly divided into a control group with 11 rats and a model building group with 55 rats.The model was fed with high-sugar and high-fat feed combined with streptozotocin to establish a T2DM model.The control group was fed with standard pellet feed and an equal volume of 0.1 mmol/L citric acid-sodium citrate buffer.The successfully modeled rats were divided into a model group,a low,medium and high concentration group of Yiqi Yangyin Huoxue Formula,and the Western medicine group,each with 11 rats.The model group and the control group were given distilled water,the low,medium and high concentration groups of Yiqi Yangyin Huoxue Formula of 0.43 g/mL,0.86 g/mL,1.72 g/mL; the Western medicine group was given 20 mg/mL metformin suspension; the intragastric dose of each group was 10 mL/kg,once a day,for 12 consecutive weeks.Fasting blood glucose(FBG),fasting insulin(FINS),insulin secretion index(HOMA-β),islet cell apoptosis index,islet microribonucleic acid-375(miR-375) and B lymphoma-2(Bcl-2) The expression level of Caspase-3 containing cysteine were tested and compared.Results:Compared with the model control group,the levels of FBG,apoptotic index of islet cells,miR-375 and Caspase-3 expression levels in islet tissues were lower,the levels of FINS and HOMA-β,and Bcl-2 expression levels in islet tissues were higher in each group,the differences were all statistically significant(P<0.05),and Yiqi Yangyin Huoxue Formula group showed a certain dose effect relationship.Conclusion:Yiqi Yangyin Huoxue Formula can reduce blood sugar level in rats with type 2 diabetes mellitus in a dose-dependent manner,improve islet secretion function and inhibit islet cell apoptosis,the effect may be related to inhibiting the expression of miR-375 in islet tissue,and then regulating the expression of apoptosis-related proteins Bcl-2 and Caspase-3. |
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