| 引用本文:郭光鑫1，孙颖1，栾海鹏2，管东辉3.基于网药学方法探究透脓散治疗慢性骨髓炎的分子作用机制[J].世界中医药,2022,(06):. |
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| 基于网药学方法探究透脓散治疗慢性骨髓炎的分子作用机制 |
| Exploration of Molecular Action Mechanism of Tounong Powder in the Treatment of Chronic Osteomyelitis Based on Pharmacy Network |
| 投稿时间：2020-07-28 |
| DOI：10.3969/j.issn.1673-7202.2022.06.004 |
| 中文关键词: 透脓散 网络药理学 慢性骨髓炎 信号通路 作用机制 |
| English Keywords:Touyu Powder Network pharmacology Chronic osteomyelitis Signaling pathway Mechanism action |
| 基金项目:山东省中医药科技发展计划项目(2019-0144) |
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| 中文摘要: |
| 目的：使用中药复方网络药理学技术探讨透脓散治疗慢性骨髓炎的潜在作用机制。方法：通过中药系统药理学数据库与分析平台(TCMSP）数据库搜索透脓散中黄芪、当归、川芎、皂角刺中所含有的化合物（因目前国家对于穿山甲稀有动物的保护，为响应国家号召，未进行穿山甲化合物的采集），筛选出透脓散中药物的符合要求的活性成分及作用靶点；通过人类基因数据库（GeneCards）搜索慢性骨髓炎的靶点信息；使用Cytoscape分析软件进行“药物-化合物-靶点”分析，导入功能蛋白联系网络数据库（STRING）行蛋白质-蛋白质相互作用（PPI）网络的分析，之后使用DAVID数据库进行Gene ID转换，差异基因的功能、通路富集分析等。结果：筛选后共获得透脓散治疗慢性骨髓炎的431个相关成分和227个靶点信息；慢性骨髓炎的相关靶点信息438个；PPI网络46个核心蛋白，关键涉及白细胞介素-10、白细胞介素-6、MMP9、TP53等；获得GO生物过程368条，主要涉及细胞增殖、免疫、炎症反应、细胞凋亡；KEGG信号通路88条，涉及破骨细胞分化、疟疾、甲型流感、类风湿性关节炎、核因子κB以及TNF信号通路。结论：透脓散可能通过控制细胞凋亡、免疫炎症反应等途径对慢性骨髓炎发挥治疗作用，通过分析为透脓散治疗慢性骨髓炎提供微观层面的理论信息支持。 |
| English Summary: |
| To explore the potential action mechanism of Touyu Powder in the treatment of chronic osteomyelitis by using Chinese herbal compound network pharmacology technology.Methods:Through the database of the Laboratory of Computational Systems Biology(TCMSP)，we searched for compounds contained in Astragalus，Angelica，Ligusticum chuanxiong and Saponaria japonicus in Toupu Powder(Due to the current national protection of rare pangolin animals，in response to the call of the country，no collection of pangolin compounds has been carried out).We screened out the active ingredients and target of action of the drugs in Touyu Powder; we searched for the target information of chronic osteomyelitis through the human gene database(GeneCards); used Cytoscape analysis software to perform “drug-compound-target” analysis，imported the functional protein connection network database(STRING) to analyze the protein interaction network，and then used the DAVID database for Gene ID conversion，differential gene function，pathway enrichment analysis，etc.Results:After screening，a total of 431 related components and 227 target information for the treatment of chronic osteomyelitis by Touyu Powder were obtained; related target information for chronic osteomyelitis was 438; there were 46 core proteins of protein interaction network.The key was related to interleukin 10，interleukin 6，and interleukin 6 MMP9，TP53，etc.; we obtained 368 GO biological processes，mainly related to cell proliferation，immunity，inflammation，apoptosis; there were 88 KEGG signaling pathways，related to osteoclast differentiation，malaria，influenza A，rheumatoid arthritis，NF-kB and TNF signaling pathway.Conclusion:Touyu Powder may have a therapeutic effect on chronic osteomyelitis by controlling cell apoptosis and immune inflammatory response.The analysis of this paper provides microscopic theoretical information support for the treatment of chronic osteomyelitis with Touyu Powder. |
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