| 引用本文:陈润铭1，杜玉青2，李友山1，刘亚莉3，刘凤桐1，谢存香1，安琪1，董学宇1.复方黄柏液对内皮细胞增殖迁移成管影响的机制研究[J].世界中医药,2022,(06):. |
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| 复方黄柏液对内皮细胞增殖迁移成管影响的机制研究 |
| Effect and Mechanism of Fufang Huangbai Liquid on Proliferation,Migration,and Tube Formation of Endothelial Cells |
| 投稿时间：2020-03-03 |
| DOI：10.3969/j.issn.1673-7202.2022.06.006 |
| 中文关键词: 复方黄柏液 中药复方制剂 内皮细胞 增殖 迁移 成管 机制 糖尿病足溃疡 |
| English Keywords:Fufang Huangbai Liquid Chinese medicinal compound preparations Endothelial cell Proliferation Migration Tube formation Mechanism Diabetic foot ulcer |
| 基金项目:国家自然科学基金面上项目(81973849-H2709)——基于AGEs-bFGF/PDGF-β通路研究中医外治“溻渍法”促进糖尿病足溃疡愈合的分子机制;北京中医药大学2017“青苗人才”计划项目(DZMYS-201701)——中医外治“溻渍法”(复方黄柏液涂剂)调控血管新生作用及其机制研究;北京中医药大学基本科研业务费项目(2016-JYB-JSPY-018)——中药外治干预大鼠自体骨髓干细胞移植治疗糖尿病溃疡对miRNA-146a的影响 |
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| 中文摘要: |
| 目的:探索复方黄柏液对内皮细胞增殖迁移成管影响的机制,为复方黄柏溶液用于治疗糖尿病足溃疡提供依据。方法:用CCK-8法检测药物毒性，探索复方黄柏液使用的最佳药物浓度、CCK-8法检测细胞增殖活力、细胞划痕实验检测细胞迁移率、成管实验检测细胞成管能力、蛋白质印迹法(Western Blotting)实验检测蛋白表达水平的变化。结果:使用复方黄柏液后,高糖组内皮细胞的增殖、迁移、成管能力较前有了提高,并降低了促血管生成素2(Ang-2)蛋白水平,增加血管内皮生长因子(VEGF)和磷酸化酪氨酸激酶受体-2(P-Tie)蛋白水平。结论:复方黄柏液通过促进内皮细胞增殖、迁移、成管以及影响蛋白表达水平,改善内皮功能障碍来实现对糖尿病足溃疡的恢复。 |
| English Summary: |
| To explore the effect and mechanism of Fufang Huangbai Liquid on endothelial cell proliferation,migration,and tube formation and provide evidence for the treatment of diabetic foot ulcer by Fufang Huangbai Liquid.Methods:Cell Counting Kit-8(CCK-8) was used for the detection of drug toxicity to determine the optimal concentration of Fufang Huangbai Liquid.Cell proliferation activity was detected by CCK-8 assay,cell migration rate by wound healing assay,tube formation ability by tube formation assay,and protein expression changes by Western blot.Results:After Fufang Huangbai Liquid treatment,the proliferation,migration,and tube formation abilities of endothelial cells in the high-glucose group were potentiated.Furthermore,the angiopoietin 2(Ang2) protein expression was reduced,and the protein expression levels of vascular endothelial growth factor(VEGF) and phosphorylated tyrosine kinase receptor-2(p-Tie2) were up-regulated.Conclusion:Fufang Huangbai Liquid can relieve diabetic foot ulcer by promoting endothelial cell proliferation,migration,and tube formation,affecting protein expression,and improving endothelial dysfunction. |
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