| To study the mechanism of Jinzhao Capsules in the protection against acute alcoholic liver injury and provide experimental evidence for the clinical application of the capsules.Methods:The Institute of Cancer Research(ICR) mice were randomly assigned into a blank group,a model group,a positive drug group,and low-,medium-,and high-dose Jinzhao Capsules groups,with 10 mice in each group.After 4 weeks of administration,50% ethanol was used to establish the mouse model of acute alcoholic liver injury.The state of mice was observed,and the drunken state,serum transaminase activity,activities of the enzymes associated with liver tissue ethanol and fat metabolism,and activities of antioxidant enzymes in the liver tissue and stomach tissue were determined.The liver samples were collected for pathological examination via hematoxylin-eosin(HE) staining.Results:Compared with the model group,Jinzhao Capsules weakened the activities of glutamic-pyruvic transaminase(GPT) and glutamic-oxaloacetic transaminase(GOT) in the serum(P<0.01,P<0.05),lowered the levels of total cholesterol(TC),triacylglycerol(TG),and malondialdehyde(MDA) in the liver tissue(P<0.01,P<0.05),increased the activities of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH),alcohol dehydrogenase(ADH),and aldehyde dehydrogenase(ALDH)(P<0.01,P<0.05),and reduced the liver cell necrosis.Furthermore,the capsules lowered the level of MDA in stomach tissue(P<0.01) and elevated the levels of SOD,nitric oxide(NO),and prostaglandin E2(PGE2)(P<0.01,P<0.05).Conclusion:Jinzhao Capsules may protect mice from acute alcoholic liver injury by enhancing the activity of key enzymes in alcohol metabolism and antioxidant enzymes in the liver tissue,inhibiting the production of lipid peroxides,protecting the integrity of liver cell membrane,and reducing the release of transaminase.At the same time,Jinzhao Capsules can protect the gastric mucosa by regulating the activity of peroxidase in the gastric tissue. |
