| 引用本文:李国卫，罗宇琴，刘潇晗，邱韵静，刘晓琳，吴文平，索彩仙，孙冬梅.不同产地矮地茶药材差异成分筛选及标准汤剂的质量研究[J].世界中医药,2023,(04):. |
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| 不同产地矮地茶药材差异成分筛选及标准汤剂的质量研究 |
| Screening of Differential Components in Herba Ardisiae Japonicae of Different Origins and Quality of Its Standard Decoctions |
| 投稿时间：2022-07-25 |
| DOI：10.3969/j.issn.1673-7202.2023.04.001 |
| 中文关键词: 矮地茶 岩白菜素 槲皮苷 标准汤剂 判别分析 熵权Topsis法 量值传递 质量控制 |
| English Keywords:Herba Ardisiae Japonicae Bergenin Quercetin Standard decoction Discriminant analysis Entropy-weighted TOPSIS method Dissemination of quantity value Quality control |
| 基金项目:国家工业和信息化部2019年产业技术基础公共服务平台项目（2019-00902-1-2） |
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| 中文摘要: |
| 目的：建立矮地茶超高效液相色谱法特征图谱以筛选不同产地矮地茶药材差异成分，并同时建立岩白菜素和槲皮苷的含量测定方法以探索饮片及标准汤剂的量值传递。方法：制备矮地茶标准汤剂，同时建立超高效液相色谱法特征图谱及含量测定方法，对不同产地的17批矮地茶样品进行测定，采用判别分析及熵权Topsis法对不同产地矮地茶药材进行评价，筛选其差异化合物，并对其中2个成分进行含量测定。结果：通过建立矮地茶超高效液相色谱法特征图谱指认了4个成分；筛选得到不同产地间的矮地茶样品差异化合物为峰2、峰4（岩白菜素）、峰5（杨梅苷）。标准汤剂岩白菜素含量均值为4.53%，转移率均值为63.05%，槲皮苷含量均值为0.57%，转移率均值为57.31%，量值传递均较好。结论：本研究采用超高效液相色谱法特征图谱结合多指标含量测定评价不同产地矮地茶药材和标准汤剂量值传递规律，结果科学合理，为矮地茶药材和标准汤剂的质量控制及评价提供参考。 |
| English Summary: |
| To screen the differential components in Herba Ardisiae Japonicae of different origins by the ultra-high-performance liquid chromatography(UPLC)，and to construct a method for the determination of bergenin and quercetin to explore the dissemination of quantity value between decoction pieces and standard decoctions.Methods:Standard decoction of Herba Ardisiae Japonicae was prepared.The UPLC chromatogram and the content determination method were established to determine 17 batches of Herba Ardisiae Japonicae samples from different producing areas.Discriminant analysis and entropy-weighted TOPSIS method were performed to evaluate the samples and screen the differential compounds，and the contents of two components were measured.Results:Four components were identified，and the screened differential compounds in Herba Ardisiae Japonicae of different origins were peak 2，peak 4(bergenin)，and peak 5(myricitrin).In the standard decoction，the average contents of bergenin and quercetin were 4.53% and 0.57%，respectively，and the average transfer rates were 63.05% and 57.31%，respectively，indicating good dissemination of quantity value.Conclusion:In this study，UPLC chromatogram combined with multi-index content determination was used to evaluate the dissemination law of quantity value between Herba Ardisiae Japonicae from different producing areas and its standard decoctions，and achieves scientific and reasonable results.This study provides a reference for the quality control and evaluation of Herba Ardisiae Japonicae and its standard decoction. |
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