| 引用本文:南海鹏1，刘金莲2，陈亚文1，陶然1，邸松蕊2，张建军2，王淳2，陈洪璋3，李波4，王林元1.基于超高效液相色谱-四级杆-飞行时间质谱技术分析清利湿热颗粒化学成分[J].世界中医药,2023,(06):. |
|
| 基于超高效液相色谱-四级杆-飞行时间质谱技术分析清利湿热颗粒化学成分 |
| Chemical Component Analysis of Qingli Shire Granules Based on Ultra-performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry |
| 投稿时间：2022-10-15 |
| DOI：10.3969/j.issn.1673-7202.2023.06.002 |
| 中文关键词: 清利湿热颗粒 化学成分 超高效液相色谱-四级杆-飞行时间质谱 京尼平苷酸 毛蕊花糖苷 杯苋甾酮 药效物质 |
| English Keywords:Qingli Shire Granules Components UPLC-Q-TOF-MS Geniposidic acid Acteoside Cyasterone Medicinal substances |
| 基金项目:国家重点研发计划项目（2018YFC1706800） |
|
| 摘要点击次数: 559 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：采用超高效液相色谱-四级杆-飞行时间质谱（UPLC-Q-TOF-MS）技术初步鉴定经水提、浓缩、干燥、制粒后的清利湿热颗粒中的化学成分并确定其药味归属。方法：采用Agilent ZORBAX RRHD Eclipse XDB-C18色谱柱（2.1 mm×100 mm，1.8 μm），以0.1%甲酸水溶液-乙腈为流动相体系，梯度洗脱，进样量为5 μL，在正、负离子模式下对处理后的清利湿热颗粒进行质谱检测。结果：通过与Natural Products HR-MS/MS Spectral Library 1.0数据库对比、文献分析以及质谱裂解规律从清利湿热颗粒样品中鉴定出54个化合物。结论：本研究为清利湿热颗粒质量控制及确定该产品可能的药效物质基础提供了依据。 |
| English Summary: |
| To identify the chemical components of Qingli Shire Granules after water extraction，concentration，drying and granulation and determine the attribution of their medicinal flavor by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Methods:The determination was performed on an Agilent ZORBAX RRHD Eclipse XDB-C18 column(2.1 mm×100 mm，1.8 μm) with gradient elution of 0.1% formic acid aqueous solution and acetonitrile as mobile phase system.The sample size was 5 μL.The treated Qingli Shire Granules were detected by mass spectrometry under positive and negative ion modes.Results:Fifty-four compounds were identified from the samples by comparing with Natural Products HR-MS/MS Spectral Library 1.0 database，literature analysis，and cracking rules of mass spectrometry.Conclusion:This study provides a basis for the quality control of Qingli Shire Granules and preliminarily determines the possible pharmacodynamic substance basis of this product. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
