| 引用本文:齐心1，王雪峰2，赫昊1.小儿解表口服液体外抗流感病毒作用的研究[J].世界中医药,2023,(06):. |
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| 小儿解表口服液体外抗流感病毒作用的研究 |
| Xiao'er Jiebiao Oral Liquid Against Influenza Virus In Vitro |
| 投稿时间：2022-03-29 |
| DOI：10.3969/j.issn.1673-7202.2023.06.006 |
| 中文关键词: 小儿解表口服液 流感病毒 细胞实验 MTT法 间接免疫荧光 抗病毒指数 神经氨酸酶 Ⅰ型干扰素 |
| English Keywords:Xiao'er Jiebiao Oral Liquid Influenza virus Cell experiment MTT method Indirect immunofluorescence Antiviral index Neuraminidase Type Ⅰ interferon |
| 基金项目:国家自然科学基金项目(81973907)——基于“脏病及腑”理论探讨宣白承气汤对Ⅳ与SPN共感染致肺肠免疫损伤及ROR调控机制研究；国家中医药管理局中医药循证能力建设项目（2019XZZX-LG006）——儿童流感中医防治方案循证评价研究 |
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| 中文摘要: |
| 目的：研究小儿解表口服液对感染H1N1的A549细胞的疗效及可能的药效机制。方法:通过不同浓度的小儿解表口服液对A549细胞的毒性实验及抗病毒实验，并采用MTT法结合CPE法得出药物的半数细胞毒性浓度（TC50）、半数细胞抑制浓度（IC50）及治疗指数（TI）。应用间接免疫荧光法检测小儿解表口服液作用于感染H1N1的A549细胞神经氨酸酶的变化。利用酶联免疫吸附试验检测小儿解表口服液对A549病毒模型干扰素-α（IFN-α）、IFN-β表达的影响。结果:小儿解表口服液TC50=2.31 g/L，IC50=0.49 g/L，TI=4.71。与模型组比较，小儿解表口服液组在24 h、48 h、72 h均减少流感病毒神经氨酸酶的表达。在24 h、48 h、72 h3个时间点中，与模型组比较，小儿解表口服液组均减少A549 IFN-α、IFN-β的释放，差异有统计学意义（均P<0.05）。结论:小儿解表口服液对感染H1N1的A549有疗效，并且其降低IFN-α、IFN-β的表达，可能是产生疗效的作用机制。 |
| English Summary: |
| To study the efficacy and possible pharmacodynamic mechanism of Xiao'er Jiebiao Oral Liquid in A549 infected with H1N1.Methods:The cytotoxicity test and antiviral test of Xiao'er Jiebiao Oral Liquid with different concentrations on A549 were carried out，and the half cytotoxic concentration(TC50)，half cell inhibition concentration(IC50)，and therapeutic index(TI) of the drug were obtained by the MTT method combined with the CPE method.The indirect immunofluorescence method was used to detect the changes in neuraminidase after Xiao'er Jiebiao Oral Liquid acted on A549 infected with H1N1.The effect of Xiao'er Jiebiao Oral Liquid on the expression of interferon-α(IFN-α) and interferon-β(IFN-β) in the A549 virus model was detected by enzyme-linked immunosorbent assay.Results:It was shown that TC50=2.31 g/L，IC50=0.49 g/L，and TI=4.71 in Xiao'er Jiebiao Oral Liquid.Compared with the model group，the Xiao'er Jiebiao Oral Liquid group reduced the expression of influenza virus neuraminidase at 24 h，48 h，and 72 h.At 24 h，48 h，and 72 h，the release of A549 IFN-α and IFN-β in the Xiao'er Jiebiao Oral Liquid group was reduced compared with the model group(P<0.05).Conclusion:Xiao'er Jiebiao Oral Liquid has a curative effect on A549 infected with H1N1，reducing the expression of IFN-α and IFN-β，which may be the mechanism of the curative effect. |
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