| 引用本文:徐敏1，程迎迎1，李鹏飞1，蒙飞1，胡堇叶2，吴倩2，赵苏瑛1.黄芪皂苷对不同类型乳腺癌细胞的作用效应及机制研究[J].世界中医药,2023,(06):. |
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| 黄芪皂苷对不同类型乳腺癌细胞的作用效应及机制研究 |
| Effects and Mechanisms of Astragaloside in the Treatment of Different Breast Cancer Cell Lines |
| 投稿时间：2021-06-16 |
| DOI：10.3969/j.issn.1673-7202.2023.06.008 |
| 中文关键词: 黄芪皂苷 中药 乳腺癌 机制 效应 抑制 凋亡 抗肿瘤 |
| English Keywords:Astragaloside Chinese medicine Breast cancer Mechanism Effect Inhibition Apoptosis Anti-tumor |
| 基金项目:江苏省中医药局科技项目（LZ13052）；江苏省中医院“益中”项目（2019Y48） |
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| 中文摘要: |
| 目的：研究中药黄芪皂苷对不同类型乳腺癌细胞的作用效应及对胞内miRNA-155表达的影响，探讨其发挥效应可能的途径，为阐明中药的抗肿瘤作用机制和其临床应用的推广提供理论依据。方法：选择代表不同类型的乳腺癌细胞系MCF-7，MDA-MB-231和T-47D，选取对数生长期的细胞随机分为对照组和加药组，在加药组中加入不同浓度的黄芪皂苷溶液（终浓度0 μg/mL、12.5 μg/mL、25 μg/mL、50 μg/mL、100 μg/mL），绘制细胞生长曲线并检测细胞克隆形成情况，并用流式细胞仪检测细胞凋亡，使用qRT-PCR测定FOX3a、SOCS1的mRNA表达水平。结果：黄芪皂苷在体外能抑制3种乳腺癌细胞系的生长和增殖，并且诱导乳腺癌细胞凋亡，但作用效应不尽相同。对T-47D的作用最强并且呈剂量依赖型，对MDA-MB-231和MCF-7的作用稍弱并呈时间-剂量依赖型。黄芪皂苷能使MCF-7和T-47D中的FOX3a、SOCS1表达降低，而MDA-MB-231中FOX3a,SOCS1升高。结论：黄芪皂苷对乳腺癌有抑制增殖、诱导凋亡的作用，并且对不同的细胞类型作用效应不同。在MCF-7和T-47D中，黄芪皂苷通过活化JAK-STAT-SOCS和INS/IGF-1信号通路中的分子FOX3a,SOCS1来发挥效应，而在MDA-MB-231中则不然，其作用的准确途径和机制尚需进一步地深入研究。 |
| English Summary: |
| To study the effects of astragaloside on different breast cancer cell lines and the expression of miRNA-155，explore the possible pathways，and thus provide a theoretical basis for elucidating the mechanism and promoting the application of Chinese medicines in the treatment of cancers.Methods:MCF-7，MDA-MB-231，and T-47D cells in the logarithmic growth phase were randomly assigned into a control group and an observation group.Astragaloside solutions were added to reach different final concentrations(0，12.5，25，50，and 100 μg/mL) in the observation group.Cell growth curves were established and colony formation was examined.The cell apoptosis was detected by flow cytometry，and the mRNA levels of FOX3a and SOCS1 were determined by qRT-PCR.Results:Astragaloside inhibited the growth and proliferation and induced the apoptosis of the three breast cancer cell lines in vitro，demonstrating the strongest effect on T-47D cells in a dose-dependent manner and weaker effects on MDA-MB-231 and MCF-7 cells in a time-and dose-dependent manner.Astragaloside down-regulated the expression levels of FOX3a and SOCS1 in MCF-7 and T-47D cells，while it up-regulated the expression levels of FOX3a and SOCS1 in MDA-MB-231 cells.Conclusion:Astragaloside inhibited the proliferation and induced the apoptosis of breast cancer cells，with varied effects on different cell lines.Astragaloside can activate the expression of FOX3a and SOCS1 via JAK-STAT-SOCS and INS/IGF-1 signaling pathways in MCF-7 and T-47D cells，but not in MDA-MB-231 cells.The specific mechanism remains to be studied. |
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