| 引用本文:刘正1，郑永仁2，陈致宏1，陈妍蓓1，朱笑举1，尹梅芳1，潘锋1.电针“梨状二穴”治疗腰椎间盘突出症大鼠的实验研究[J].世界中医药,2023,(06):. |
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| 电针“梨状二穴”治疗腰椎间盘突出症大鼠的实验研究 |
| Electroacupuncture at Two Points of Piriform Muscle for Lumbar Disc Herniation in Model Rats |
| 投稿时间：2021-06-22 |
| DOI：10.3969/j.issn.1673-7202.2023.06.010 |
| 中文关键词: 电针 梨状二穴 腰椎间盘突出症 大鼠 白细胞介素-6 一氧化氮 前列腺素E2 基质金属蛋白酶-3 |
| English Keywords:Electroacupuncture Two points of piriform muscle Lumbar disc herniation Rats IL-6 NO PGE2 MMP-3 |
| 基金项目:2017年云南省科学技术厅-云南中医学院应用基础研究联合专项-中医联合面上项目［2017FF117（-052)］——“梨状二穴”治疗腰椎间盘突出神经根压迫症状大鼠的实验研究 |
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| 中文摘要: |
| 目的：探讨电针“梨状二穴”对腰椎间盘突出症大鼠血清白细胞介素-6（IL-6）、一氧化氮（NO）、前列腺素E2（PGE2）、基质金属蛋白酶-3（MMP-3）等炎症介质表达的影响。方法：将32只SD大鼠随机分为空白组、模型组、电针1组、电针2组。除空白组外，其余3组均按照非压迫性腰椎间盘髓核突出模型的造模方法进行造模。电针1组、电针2组分别给予相应干预措施，在造模结束以及治疗后的第1、3、6天对大鼠后肢热刺激缩爪阈差值进行检测，治疗后的第6天用酶联免疫吸附试验（ELISA）测定血清标本中IL-6、NO、PGE2、MMP-3的水平。结果：造模后第3天，与空白组比较，模型组、电针1组、电针2组大鼠的缩爪阈值明显下降（P<0.01）；治疗后第6天，模型组治疗前后缩爪阈值比较，差异无统计学意义（P>0.05）；电针1组、电针2组治疗前后比较，缩爪阈值明显升高，差异均有统计学意义（均P<0.01）。治疗后电针1组、电针2组IL-6、NO、PGE2、MMP-3水平明显低于模型组，差异均有统计学意义（均P<0.01）。结论：电针“梨状二穴”降低了腰椎间盘突出症大鼠IL-6、NO、PGE2、MMP-3的表达水平，从而起到抗炎镇痛的作用。 |
| English Summary: |
| To investigate the effects of electroacupuncture at two points of piriform muscle on the expression of interleukin-6(IL-6)，nitric oxide(NO)，prostaglandin 2(PGE2)，and matrix metalloproteinase 3(MMP-3) in the serum of model rats of lumbar disc herniation.Methods:A total of 32 SD rats were randomly assigned into a blank group，a model group，and electroacupuncture groups 1 and 2，with 8 rats in each group.Except the blank group，the other 3 groups were modeled according to the modeling method of non-compressive model of nucleus pulposus of lumbar intervertebral disc.Electroacupuncture groups 1 and 2 were treated with the corresponding intervention measures.At the end of modeling and on the 1st，3rd，and 6th days of treatment，the hind paw withdrawal threshold was measured.The serum levels of IL-6，NO，PGE2，and MMP-3 were measured by enzyme-linked immunosorbent assay(ELISA) after 6 days of treatment.Results:Three days after modeling，the paw withdrawal thresholds in the model group and electroacupuncture groups 1 and 2 declined compared with that in the blank group(P<0.01).The paw withdrawal threshold in the model group showed no significant difference before and after 6 days of treatment(P>0.05).The electroacupuncture increased the paw withdrawal threshold compared with that before treatment(P<0.01).The levels of IL-6，NO，PGE2，and MMP-3 in electroacupuncture groups 1 and 2 were lower than those in the model group(P<0.01).Conclusion:Electroacupuncture at two points of piriform muscle can down-regulate the expression of IL-6，NO，PGE2，and MMP-3 in the serum to exert the anti-inflammatory and pain-relieving effects on lumbar disc herniation in model rats. |
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