| 引用本文:洪倩1，马增春2，王增慧1，韩莉1，王青1，杨阳1.小儿柴桂退热口服液对发热模型大鼠的退热作用和机制研究[J].世界中医药,2023,(11):. |
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| 小儿柴桂退热口服液对发热模型大鼠的退热作用和机制研究 |
| Antipyretic Effect and Mechanism of Xiaoer Chaigui Tuire Oral Liquid on Fever Model Rats |
| 投稿时间：2021-11-18 |
| DOI：10.3969/j.issn.1673-7202.2023.11.012 |
| 中文关键词: 小儿柴桂退热口服液 退热作用 退热机制 发热模型大鼠 酵母 体温 炎症介质 下丘脑发热中枢 |
| English Keywords:Xiaoer Chaigui Tuire Oral Liquid Antipyretic effect Antipyretic mechanism Fever model rat Yeast Body temperature Inflammatory mediator Hypothalamic fever central |
| 基金项目:国家中医药管理局国家中医药传承创新团队项目（ZYYCXTD-C-202009） |
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| 中文摘要: |
| 目的：研究小儿柴桂退热口服液对酵母发热模型大鼠的退热作用机制。方法：将45只雄性SD大鼠皮下注射20%酵母混悬液，制备酵母发热模型大鼠，通过随机数字表随机分为模型组、布洛芬组、柴桂低剂量组、柴桂中剂量组、柴桂高剂量组，每组9只，模型组给予0.75 mL/kg剂量生理盐水溶液灌胃，布洛芬组给予0.75 mL/kg剂量右旋布洛芬口服混悬液灌胃，柴桂各剂量组分别给予1.25 mL/kg、2.5 mL/kg、5 mL/kg剂量小儿柴桂退热口服液灌胃；另取9只未造模大鼠为空白组，给予0.75 mL/kg剂量生理盐水溶液灌胃。给药1 h、2 h后，比较各组大鼠体温变化情况；给药2 h后，比较各组大鼠血清白细胞介素-1β（IL-1β）、一氧化氮（NO）、髓过氧化物酶（MPO）含量及下丘脑前列腺素E2（PGE2）、环磷酸腺苷（cAMP）含量。结果：与模型组比较，治疗1 h、2 h后各给药组大鼠体温升高幅度均有所降低，且随着时间延长体温下降趋势越显著。与模型组比较，布洛芬组、柴桂各剂量组大鼠IL-1β、NO、MOP含量以及PGE2、cAMP含量均显著降低（均P<0.01）；柴桂各剂量组的效应强度与剂量均正相关，但均弱于布洛芬组。结论：小儿柴桂退热口服液能够降低酵母发热模型大鼠的体温升高，且退热作用较为温和；其作用机制与抑制血清炎症介质IL-1β、NO、MOP的生成与释放，减少下丘脑发热中枢PGE2、cAMP的合成与释放有关。 |
| English Summary: |
| To study the antipyretic effect and mechanism of Xiaoer Chaigui Tuire Oral Liquid(XECGTR) on yeast-induced fever model rats.Methods:Forty-five male SD rats were subcutaneously injected with a 20% yeast suspension to establish the yeast-induced fever model.The model rats were randomly divided into a model group，an ibuprofen group，and low-，medium-，and high-dose XECGTR groups，with nine rats in each group.The model group received normal saline orally at 0.75 mL/kg，the ibuprofen group received D-ibuprofen oral suspension at 0.75 mL/kg，and the XECGTR groups received XECGTR orally at 1.25，2.5，and 5 mL/kg，respectively.Another nine rats without modeling were assigned to the blank group and received normal saline orally at 0.75 mL/kg.The changes in body temperature were compared among the groups at 1 and 2 h after administration.Two hours after administration，the levels of interleukin-1β(IL-1β)，nitric oxide(NO)，myeloperoxidase(MPO)，prostaglandin E2(PGE2)，and cyclic adenosine monophosphate(cAMP) in the serum were compared among the groups.Results:The temperature increase in each treatment group was reduced at 1 and 2 h after administration，and the temperature decrease became more significant over time as compared with that in the model group.Compared with the results in the model group，the levels of IL-1β，NO，MPO，PGE2，and cAMP in the ibuprofen group and XECGTR groups significantly decreased(all P<0.01).The efficacy of XECGTR was positively correlated with the dosage，but weaker than that of the ibuprofen group.Conclusion:XECGTR can reduce the increase in body temperature in yeast-induced fever model rats，and its antipyretic effect is relatively mild.The mechanism may be related to the inhibition of the production and release of serum inflammatory mediators IL-1β，NO，and MPO，as well as the reduction of the synthesis and release of PGE2 and cAMP in the hypothalamic fever center. |
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