| 引用本文:王芬1，何华亮2，刘铜华3，吴丽丽3.糖耐康改善KKAy小鼠Toll样受体4通路及葡萄糖转运子4的作用机制研究[J].世界中医药,2023,(19):. |
|
| 糖耐康改善KKAy小鼠Toll样受体4通路及葡萄糖转运子4的作用机制研究 |
| Mechanism of Tangnai Kang in Improving TLR4 Signaling Pathway and GluT-4 in KKAy Diabetic Mice |
| 投稿时间：2022-09-27 |
| DOI：10.3969/j.issn.1673-7202.2023.19.003 |
| 中文关键词: 糖耐康 自发性糖尿病小鼠 TLR4通路 白细胞介素6 核因子κB激酶β抑制物 葡萄糖转运蛋白4 作用机制 |
| English Keywords:Tangnai Kang Spontaneous diabetic KKAy mice Toll-like receptor 4(TLR4) signaling pathway Interleukin-6(IL-6) Inhibitor of nuclear factor kappaB kinase beta(IKKβ) Glucose transporter 4(GluT-4) Mechanism |
| 基金项目:国家自然科学基金青年科学基金项目（82004338）；教育部优秀青年科学基金项目（2018-JYBZZ-JS169） |
|
| 摘要点击次数: 446 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：观察中药糖耐康对KKAy小鼠血糖、体质量及Toll样受体4（TLR4）通路中白细胞介素6（IL-6）、核因子κB激酶β抑制物（IKKβ）及葡萄糖转运蛋白4（GluT4）影响，分析其改善2型糖尿病的可能作用机制。方法：选取无特定病原体（SPF）级自发性2型糖尿病动物KKAy小鼠及C57BL/6J小鼠，给予高脂饲料喂养，连续8周给药，评价血糖及体质量。并采用半定量酶联免疫吸附测定（ELISA）检测血浆中IL-6及GluT4的水平。蛋白质印迹法检测肝组织TLR4蛋白表达、肌肉组织IKKβ蛋白表达水平。结果：中药糖耐康高剂量组，能够显著降低血糖水平（P<0.05），具有一定抑制体质量增长的趋势。同时能够下调IL-6水平，上调GluT4的水平（P<0.01，P<0.05），抑制TLR4及IKKβ蛋白表达（P<0.01，P<0.05）。结论：糖耐康可能通过下调脂多糖（LPS）介导的TLR4通路中关键炎症介质的水平，减轻低度炎症反应，在一定程度上抑制炎症级联效应，同时提高胰岛素信号转导受体后途径中GluT4的转位及活性水平，共同达到改善胰岛素抵抗状态的作用。 |
| English Summary: |
| To observe the effects of traditional Chinese medicine(TCM) compound Tangnai Kang on blood sugar，body weight，interleukin-6(IL-6) and inhibitor of nuclear factor kappaB kinase beta(IKKβ) in Toll-like receptor 4(TLR4) signaling pathway and glucose transporter 4(GluT-4) in KKAy mice，and to discuss its possible mechanism in improving type 2 diabetes mellitus(T2DM).Methods:SPF KKAy and C57BL/6J mice(spontaneous T2DM animals) were selected and fed with high fat food.After 8 weeks，blood sugar and body weight were evaluated and the levels of IL-6 and GluT-4 in plasma were measured by semi-quantitative ELISA.The protein expressions of TLR4 in liver tissue and IKKβin muscle tissue were detected by Western blot.Results:High dose of Tangnai Kang reduced blood glucose(P<0.05) and controlled the growth trend of body weight.It also decreased the level of IL-6 and increased the level of GluT-4(P<0.01，P<0.05)，and inhibited the protein expressions of TLR4 in liver tissue and IKKβ in muscle tissue(P<0.01，P<0.05).Conclusion:Tangnai Kang may reduce the low-grade inflammation response by down-regulating the key inflammatory factors in the LPS-mediated TLR4 signaling pathway，and thus inhibit the inflammatory cascade effect to some extent.It also enhances the transposition and activity of GluT-4 in the insulin signal transduction pathway，to jointly improve the insulin resistance. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
