| 引用本文:焦存1,2，杨丽晓1，张艳1，王哲3，李亦晗3，芦现杰1.左归丸靶向C-X-C趋化因子受体4对人脐带间充质干细胞体外迁移的影响[J].世界中医药,2023,(19):. |
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| 左归丸靶向C-X-C趋化因子受体4对人脐带间充质干细胞体外迁移的影响 |
| Effect of Zuogui Pill Targeting CXCR4 on the Migration of Human Umbilical Cord Mesenchymal Stem Cells In Vitro |
| 投稿时间：2022-02-23 |
| DOI：10.3969/j.issn.1673-7202.2023.19.006 |
| 中文关键词: 左归丸 人脐带间充质干细胞 体外迁移 C-X-C趋化因子受体4 基质细胞衍生因子-1/C-X-C趋化因子受体4 普乐沙福 增殖 卵巢早衰 |
| English Keywords:Zuogui Pill Human umbilical cord mesenchymal stem cells(hUC-MSCs) Migration in vitro C-X-C chemokine receptor 4(CXCR4) Stromal cell-derived factor-1(SDF-1)/CXCR4 AMD3100 Proliferation Premature ovarian failure |
| 基金项目:国家自然科学基金项目（82004402）；山东省中医药科技发展计划项目（2019-0893） |
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| 中文摘要: |
| 目的：探讨左归丸通过靶向调控C-X-C趋化因子受体4（CXCR4）表达对人脐带间充质干细胞（hUC-MSCs）体外迁移的影响。方法：采用组织块法分离培养hUC-MSCs并进行流式鉴定。利用CCK-8检测不同浓度左归丸对hUC-MSCs增殖的影响，细胞划痕和Transwell实验分析左归丸对hUC-MSCs体外迁移的影响，实时聚合酶链反应（PCR）、蛋白印迹法和流式细胞术分析hUC-MSCs中CXCR4表达变化。结果：流式细胞术结果显示，hUC-MSCs表面高表达CD73和CD90，极低表达CD34和CD45。CCK-8结果显示，0.1、0.2 mg/mL左归丸均能明显促进hUC-MSCs增殖（P<0.05）。细胞划痕和Transwell实验结果显示，0.1、0.2 mg/mL左归丸能明显促进hUC-MSCs迁移，最佳浓度为0.2 mg/mL，CXCR4拮抗剂普乐沙福则能抑制其促迁移作用（P<0.05）。实时PCR、蛋白印迹法和流式细胞术结果显示，左归丸能上调hUC-MSCs中CXCR4 mRNA和蛋白表达水平，并促进CXCR4蛋白在细胞膜表达（P<0.05）。结论：左归丸通过上调CXCR4表达促进hUC-MSCs的体外迁移。 |
| English Summary: |
| To investigate the effect of Zuogui Pill on the migration of human umbilical cord mesenchymal stem cells(hUC-MSCs) in vitro by regulating the expression of C-X-C chemokine receptor 4(CXCR4).Methods:The hUC-MSCs were isolated through tissue block method and identified by flow cytometry.CCK-8 method was used to analyze the proliferation of hUC-MSCs treated with different concentrations of Zuogui Pill.Cell scratch and Transwell assays were performed to detect the effect of Zuogui Pill on the migration of hUC-MSCs.The expression of CXCR4 in hUC-MSCs was detected by real-time PCR，Western blot，and flow cytometry.Results:The flow cytometry showed that hUC-MSCs highly expressed CD73 and CD90，while extremely lowly expressed CD34 and CD45 on their surfaces.CCK-8 assay indicated that 0.1 and 0.2 mg/mL Zuogui Pill significantly promoted the proliferation of hUC-MSCs(P<0.05).By cell scratch and Transwell assays，it was found that 0.1 and 0.2 mg/mL(optimal concentration) Zuogui Pill enhanced the migration of hUC-MSCs，and CXCR4 antagonist AMD3100 attenuated its migration-promoting effect(P<0.05).Real-time PCR，Western blot，and flow cytometry demonstrated that Zuogui Pill up-regulated the CXCR4 mRNA and protein expression of hUC-MSCs，and promoted the expression of CXCR4 on cell surface(P<0.05).Conclusion:Zuogui Pill facilitates the migration of hUC-MSCs in vitro by up-regulating the expression of CXCR4. |
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