| 引用本文:孙文婷1，万盈盈1，杨家煕2，王文乾2，王皓男1，叶婉婷2，刘宇1，寇秋爱1，郑蕊1.不同制备工艺的坤痹消方治疗类风湿关节炎的药效学研究[J].世界中医药,2024,(09):. |
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| 不同制备工艺的坤痹消方治疗类风湿关节炎的药效学研究 |
| Pharmacodynamics of Kunbixiao Prescription Prepared with Different Processes in Treating Rheumatoid Arthritis |
| 投稿时间：2022-11-07 |
| DOI：10.3969/j.issn.1673-7202.2024.09.007 |
| 中文关键词: 坤痹消方 工艺 类风湿关节炎 动物模型 炎症 病理损伤 镇痛 药效学 |
| English Keywords:Kunbixiao Prescription Process Rheumatoid arthritis Animal model Inflammation Pathological injury Relieving pain Pharmacodynamics |
| 基金项目:国家重点研发计划项目（2018YFC1706805）；中国中医科学院优势病种医院制剂新药研发专项（ZZ15-XY-PT-07） |
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| 中文摘要: |
| 目的：筛选坤痹消方治疗类风湿关节炎（RA）的最佳制备工艺。方法：根据制备方法的不同，将坤痹消方分为3种制备工艺。建立雄性及雌性牛Ⅱ型胶原诱导关节炎（CIA）大鼠模型，通过足肿胀体积、关节炎指数、脾脏指数、膝关节病理评估不同工艺的坤痹消方对RA的治疗作用。建立雄性及雌性小鼠醋酸扭体疼痛模型，通过扭体次数评估不同工艺的坤痹消方的镇痛作用。结果：坤痹消方工艺2能够显著降低雄性及雌性CIA大鼠足肿胀体积、关节炎指数（P<0.01；P<0.05）；坤痹消方工艺2、工艺3均能降低雌性CIA大鼠脾脏指数（P<0.01；P<0.05）；在病理损伤方面，坤痹消方工艺2能够显著改善雄性及雌性CIA大鼠滑膜增生及炎症细胞浸润程度（P<0.05；P<0.01）。在镇痛方面，坤痹消方工艺2能显著降低雄性小鼠扭体次数（P<0.01）；坤痹消方工艺3能显著降低雄性及雌性小鼠扭体次数（P<0.05）。结论：坤痹消方工艺2能够改善CIA大鼠关节炎症，减轻膝关节的病理损伤，并有一定的镇痛作用，为坤痹消方的最优工艺。 |
| English Summary: |
| To explore the optimum preparation process of Kunbixiao Prescription in treating rheumatoid arthritis(RA).Methods:Kunbixiao Prescription was prepared with three different processes.The male and female rat models of collagen-induced arthritis(CIA) were induced by injection of bovine type Ⅱ collagen with complete Freund's adjuvant.The paw swelling volume，arthritis index，spleen index，and pathological changes in the knee joint were estimated as the indicators of the therapeutic effects on both male and female rats in different groups.The male and female mouse models of writhing-induced pain was established with acetic acid，and the pain-relieving effects of Kunbixiao Prescription prepared with different processes on both male and female mice were evaluated.Results:The Kunbixiao Prescription prepared with process 2 reduced the paw swelling volume and arthritis index in both male and female CIA rats(P<0.01; P<0.05).The Kunbixiao Prescription prepared with processes 2 and 3 reduced the spleen index of female CIA rats(P<0.01; P<0.05).The Kunbixiao Prescription prepared with process 2 mitigated synovial hyperplasia and inflammatory infiltration in male and female CIA rats(P<0.05; P<0.01) and reduced the times of writhing in male mice(P<0.01).The Kunbixiao Prescription prepared with process 3 reduced the times of writhing in both male and female mice(P<0.05).Conclusion:The Kunbixiao Prescription prepared with process 2 ameliorated the joint inflammation and pathological changes and relieved the pain in CIA rats.This study provides a basis for the following research on the preparation process of Kunbixiao Prescription. |
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