世界中医药

基金项目:国家重点研发计划中医药现代化研究重点专项（2018YFC1706800）；国家中医药管理局国际合作司中医药国际合作专项（基地类项目）（0730-236132ZC0054/01~03）；国家中医药管理局国际合作司中医药国际合作专项（中心类项目）（0730-236132ZC0052/01~04）

作者	单位
郑婷1，梁唯灿1，吕瑞琳1，王禅缔1，乐娜1，张建军1，王林元1，陈亮2，王淳1，郭宝林3	1 北京中医药大学，北京，100029； 2 安利〔上海〕科技发展有限公司，上海，201203； 3 中国医学科学院北京协和医学院药用植物研究所，北京，100005

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中文摘要:

目的：考察紫锥菊水提物对虚寒、虚热证小鼠内分泌激素、物质能量代谢水平的影响，探讨紫锥菊水提物的药性。方法：将KM雄性小鼠随机分为空白组、虚热模型组、虚热西洋参组、虚热紫锥菊水提物低剂量组、虚热紫锥菊水提物高剂量组、虚寒模型组、虚寒人参组、虚寒紫锥菊水提物低剂量组、虚寒紫锥菊水提物高剂量组；虚热模型组小鼠每天灌胃甲状腺片水溶液（160 mg/kg），虚寒模型组小鼠灌胃氢化可的松溶液（25 mg/kg），1次/d，连续14 d制备虚热、虚寒模型，各给药组灌胃相应药物。检测各组小鼠血清三碘甲状腺原氨酸(T3)、甲状腺素(T4)、总胆固醇(TC)、三酰甘油(TG)、总蛋白(TP)、白蛋白(ALB)以及血糖(GLU)等指标变化，比色法检测小鼠肝脏乳酸脱氢酶（LDH）、琥珀酸脱氢酶（SDH）、Na+-K+-ATP酶活力，采用实时聚合酶链反应法检测小鼠肝组织细胞色素c氧化酶7A2（COX7A2）、琥珀酸脱氢酶复合体D亚基（SDHD）mRNA表达情况。结果：与虚热模型组比较，紫锥菊水提物对虚热小鼠整体状态有一定程度改善，体质量和脾脏指数有所改善（P<0.05），T3、T4水平显著降低（P<0.05，P<0.01），TP、ALB、GLU、LDH的水平显著升高（P<0.01），SDH、Na+-K+-ATP酶、COX7A2、SDHD mRNA的表达水平显著降低（P<0.05，P<0.01），紫锥菊水提物与寒凉性质的西洋参表现出相似的作用；与虚寒模型组比较，紫锥菊水提物对虚寒小鼠整体状态、胸腺指数以及脾脏指数无明显改善，紫锥菊水提物高剂量显著升高SDH、Na+-K+-ATP酶水平（P<0.01），而紫锥菊水提物对虚寒小鼠T3、T4、TP、ALB、TC、TG、GLU、COX7A2 mRNA、SDHD mRNA表达水平的调节不明显，差异无统计学意义（P>0.05），紫锥菊水提物与温热性质的人参作用趋于相反。结论：紫锥菊水提物性凉，可能是紫锥菊寒凉药性的物质基础。

English Summary:

To investigate the effects of water extract of Echinacea purpurea on the endocrine hormones and material and energy metabolism in mice with deficiency-cold and deficiency-heat syndrome and explore the pharmacological characteristics of E.purpurea.Methods:Male KM mice were randomly divided into blank group,deficiency-heat model group,deficiency-heat Panax quinquefolius group,deficiency-heat low-dose E.purpurea extract group,deficiency-heat high-dose E.purpurea extract group,deficiency-cold model group,deficiency-cold P.ginseng group,deficiency-cold low-dose E.purpurea extract group,and deficiency-cold high-dose E.purpurea extract group.Mice in the deficiency-heat model group were administered water solution of thyroid tablets(160 mg/kg) orally daily,while those in the deficiency-cold model group received hydrocortisone solution(25 mg/kg) orally once daily for 14 consecutive days to establish the deficiency-heat and deficiency-cold models,respectively.Each treatment group received corresponding drugs orally.Serum levels of triiodothyronine(T3),thyroxine(T4),total cholesterol(TC),triglycerides(TG),total protein(TP),albumin(ALB),and glucose(GLU) were measured.The activities of lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),and Na+-K+-ATP in the liver were assessed using colorimetric methods.Real-time polymerase chain reaction(PCR) was used to detect mRNA expression of cytochrome c oxidase subunit 7A2(COX7A2) and succinate dehydrogenase complex subunit D(SDHD) in liver tissue.Results:Compared to the deficiency-heat model group,E.purpurea extract improved the overall condition of deficiency-heat mice,with improvements in body weight and spleen index(P<0.05).Levels of T3 and T4 were significantly reduced(P<0.05,P<0.01),while levels of TP,ALB,GLU,and LDH were significantly increased(P<0.01).Expression levels of SDH,Na+-K+-ATP,COX7A2,and SDHD mRNA were significantly decreased(P<0.05,P<0.01).E.purpurea extract exhibited similar effects to P.quinquefolius with cold-nature properties.Compared to the deficiency-cold model group,E.purpurea extract showed no significant improvement in the overall condition,thymus index,or spleen index of deficiency-cold mice.However,high-dose E.purpurea extract significantly increased SDH and Na+-K+-ATP levels(P<0.01).There was no significant modulation of T3,T4,TP,ALB,TC,TG,GLU,COX7A2 mRNA,or SDHD mRNA expression levels in deficiency-cold mice treated with E.purpurea extract(P>0.05).E.purpurea extract exhibited effects contrary to P.ginseng with warm-nature properties.Conclusion:E.purpurea extract exhibits a cool-nature property,which may be the material basis for its cool-nature pharmacological characteristics.

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