| To investigate the preventive effects and underlying mechanisms of Buyang Huanwu Decoction on a mouse model of posttraumatic epilepsy.Methods:Thirty C57BL/6 mice were randomly divided into three groups according to a random number table：sham operation group,model group,and Buyang Huanwu Decoction group,with 10 mice in each group.The mice in the model and Buyang Huanwu Decoction groups underwent cortical cutting surgery,while those in the sham operation group only had cranial drilling.Starting on the second day post-surgery,the sham operation and model groups received saline(oral gavage) daily at 10 mL/kg,while the Buyang Huanwu Decoction group received oral Buyang Huanwu Decoction(32 g/kg) daily for 10 days.On the 21st day,pentylenetetrazol(PTZ) was injected intraperitoneally.Various parameters were compared,including the PTZ dose for the first generalized seizure,latency to the first seizure,seizure grade,total number of minor seizures,duration of major seizures,body weight,histopathological changes in cortical tissue,and protein expression of interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α) in cortical tissues.Results:Compared with the sham operation group,the model group had a lower PTZ dose for the first seizure,shorter latency,longer seizure duration,more seizures,fewer cortical neurons,severe Nissl body damage,and higher protein expression of IL-6,IL-1β,and TNF-α in the cortical tissues(all P<0.05).Compared with the model group,the Buyang Huanwu Decoction group had a higher PTZ dose for the first seizure,longer latency,shorter seizure duration,fewer seizures,more cortical neurons with relatively tight arrangement,less Nissl body damage,and lower protein expression of IL-6,IL-1β,and TNF-α in the cortical tissues(all P<0.05).Although the seizure grade in the model group was higher than that in the sham operation group,the difference was not statistically significant(P>0.05).The seizure grade in the Buyang Huanwu Decoction group was lower than that in the model group,but the difference was not statistically significant(P>0.05).Conclusion:Buyang Huanwu Decoction can increase the seizure threshold in posttraumatic epilepsy mice,improve histopathological changes in cortical tissues,and promote repair and regeneration of cortical neurons.Its mechanism of action may be related to the reduction of inflammatory responses in the cortical tissues. |
