| 引用本文:习隽丽1，周超1，李子银1，石磊2.七叶皂苷对结直肠癌细胞增殖、侵袭的作用及机制研究[J].世界中医药,2024,(13):. |
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| 七叶皂苷对结直肠癌细胞增殖、侵袭的作用及机制研究 |
| Effect and Mechanism of Escin on Proliferation and Invasion of Colorectal Cancer Cells |
| 投稿时间：2023-06-06 |
| DOI：10.3969/j.issn.1673-7202.2024.13.012 |
| 中文关键词: 七叶皂苷 结直肠癌 增殖 迁移 侵袭 SRC BTK TRIO |
| English Keywords:Escin Colorectal cancer Proliferation Migration Invasion SRC BTK TRIO |
| 基金项目:国家自然科学基金面上项目（81871816）；湖北省卫生健康委员会面上项目（WJ2021M157）；湖北省自然科学基金项目（2020CFB661）；武汉市卫生健康委员会面上项目（WX20B14）；武汉大学医学部教学研究项目（2020028） |
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| 中文摘要: |
| 目的：探究七叶皂苷对氧化三甲胺（TMAO）诱导的结直肠癌细胞株增殖及迁移侵袭的影响，并探讨其作用机制。方法：取结直肠癌HCT116细胞系，分为对照组、TMAO组、观察组3组，并分别进行处理。对照组不做处理，TMAO组仅使用100 μmol/L TMAO诱导，观察组使用同剂量TMAO诱导后再进行10 μmol/L七叶皂苷治疗处理。随后使用四甲基偶氮唑盐比色(MTT)法、划痕愈合实验及细胞迁移侵袭试验技术(Transwell)实验分别检测细胞的增殖、迁移及侵袭行为，并使用蛋白质印迹（Western Blotting）实验检测SRC、pBTK、pTRIO蛋白表达情况。结果：TMAO诱导可增强HCT116增殖及迁移侵袭能力，并显著上调SRC-BTK-TRIO通路相关基因的mRNA水平，而七叶皂苷干预能抑制癌细胞增殖（P<0.01）并降低其迁移（P<0.001）、侵袭（P<0.01）能力，同时下调SRC（P<0.05）、BTK（P<0.01）、TRIO（P<0.05）蛋白的表达。结论:七叶皂苷可通过下调SRC-BTK-TRIO通路显著抑制HCT116细胞增殖及迁移侵袭能力。 |
| English Summary: |
| To investigate the effect of escin on the proliferation，migration，and invasion of colorectal cancer cell line HCT116 induced by trimethylamine N-oxide(TMAO) and to explore its mechanism of action.Methods:HCT116 colorectal cancer cells were divided into a control group，a TMAO group，and an experimental group.The control group received no treatment，the TMAO group was induced with 100 μmol/L TMAO，and the experimental group was treated with 100 μmol/L TMAO followed by 10 μmol/L escin.Cell proliferation，migration，and invasion were assessed using the MTT assay，scratch wound healing assay，and Transwell migration and invasion assays，respectively.The expression levels of SRC，pBTK，and pTRIO proteins were measured by Western blotting.Results:TMAO induction enhanced the proliferation，migration，and invasion of HCT116 cells and significantly upregulated the mRNA levels of genes in the SRC-BTK-TRIO pathway.Escin treatment inhibited cancer cell proliferation(P<0.01)，reduced their migration(P<0.001) and invasion(P<0.01) capabilities，and downregulated the expression of SRC(P<0.05)，BTK(P<0.01)，and TRIO(P<0.05) proteins.Conclusion:Escin can significantly inhibit the proliferation，migration，and invasion of HCT116 cells by downregulating the SRC-BTK-TRIO pathway. |
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