| To observe the effect of electroacupuncture combined with Wuzi Yanzong Pills on the BDNF/TrkB/CREB signaling pathway in mice with Parkinson's disease(PD).Methods:A total of 108 male C57BL/6 were randomly divided into nine groups,including the normal group,model group,electroacupuncture group,Wuzi Yanzong Pill group,combined treatment group,inhibitor group,electroacupuncture+inhibitor group,Wuzi Yanzong Pill+inhibitor group,and combined treatment+inhibitor group,with 12 mice in each group.Except for the normal group and the inhibitor group,the other groups were given 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) by intraperitoneal injection for modeling,with 15 mg/kg on the first day,20 mg/kg on the second day,and 30 mg/kg on the third to seventh days,0.2 mL each time,once a day.The intervention was performed on the first day of modeling.The electroacupuncture group was treated with electroacupuncture intervention,and the Wuzi Yanzong Pill group was fed with Wuzi Yanzong Pill solution in the morning and evening for 14 consecutive days.The inhibitor group was intraperitoneally injected with ANA-12,a TrkB inhibitor for 14 consecutive days.After the intervention,the results of the gait experiment,open field experiment,and pole climbing experiment were compared,as well as the expressions of tyrosine hydroxylase(TH),BDNF,TrkB,and CREB in substantia nigra.Results:Compared with the normal group,the model group showed obvious dyskinesia,and the average fluorescence intensity of TH in substantia nigra and the protein expression of TH,BDNF,TrkB,and CREB decreased significantly(P<0.05).Compared with that of the model group,the dyskinesia of the electroacupuncture group,Wuzi Yanzong Pill group,and combined treatment group was improved,and the average fluorescence intensity of TH in substantia nigra and the protein expression of TH,BDNF,TrkB,and CREB were increased(P>0.05),while the dyskinesia of combined treatment group was improved compared with the electroacupuncture group and Wuzi Yanzong Pill group.The expression of other indexes increased(P<0.05).Conclusion:Electroacupuncture combined with Wuzi Yanzong Pills may improve the dyskinesia of mice with PD and protect dopaminergic neurons by regulating the BDNF/TrkB/CREB signaling pathway. |
