| 引用本文:郑彦楠1，阮郑石诗1，高建平1，贾晓薇2，丁旭彦2，袁满1.人参枳椇子口服液的解酒保肝作用研究[J].世界中医药,2024,(15):. |
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| 人参枳椇子口服液的解酒保肝作用研究 |
| Anti-inebriation and Hepatoprotective Effects of Renshen Zhijuzi Oral Liquid |
| 投稿时间：2023-12-11 |
| DOI：10.3969/j.issn.1673-7202.2024.15.006 |
| 中文关键词: 人参枳椇子口服液 急性酒精性肝病 乙醇 解酒 保肝 抗氧化 乙醇代谢 |
| English Keywords:Renshen Zhijuzi Oral Liquid Acute alcoholic liver injury Ethanol Anti-inebriation Hepatoprotective Anti-oxidation Ethanol metabolism |
| 基金项目:国家自然科学基金项目（82204723）——益母草总生物碱抑制HIF-1α介导的MCs活化抗过敏性哮喘机制研究 |
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| 中文摘要: |
| 目的：研究人参枳椇子口服液（GROL）的解酒保肝作用。方法：将50只小鼠随机分空白组、模型组、GROL高剂量组、GROL低剂量组和阳性药联苯双酯对照组，每组10只。灌胃给药4周后用50%乙醇一次灌胃建立小鼠急性醉酒模型，考察GROL对小鼠基本生理活动、小鼠血清及肝组织中相关指标，观察肝组织病理切片，评价其解酒保肝作用。采用乙醇诱导AML12肝细胞损伤模型，检测GROL干预后对乙醇刺激的AML12细胞上清液中谷丙转氨酶（GPT）、谷草转氨酶（GOT）、活性氧（ROS）水平的影响。结果：GROL组小鼠醉酒时间显著延长，睡眠、醒酒时间显著缩短；血清中乙醇浓度、GPT、GOT水平显著降低；肝细胞损伤情况有所改善；GROL组肝组织中超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GSH）水平明显提高，丙二醛（MDA）含量明显降低。对体外培养的AML12肝细胞，GROL可以逆转乙醇引起的肝细胞ALT和AST活性的升高以及过度生成的ROS量。结论：GROL具有良好的防醉、解酒和保肝作用，其发挥作用的途径可能与促进体内乙醇代谢、增强内源性抗氧化酶的活性有关。 |
| English Summary: |
| To investigate the effects of Renshen Zhijuzi Oral Liquid(RZOL) on alcohol detoxification and liver protection.Methods:Fifty mice were randomly divided into a blank group，a model group，a high-dose RZOL group，a low-dose RZOL group，and a positive control group(bifendate)，with 10 mice in each group.After 4 weeks of oral gavage，an acute alcohol intoxication model was induced using a single dose of 50% ethanol.The effects of RZOL on basic physiological activities and serum and liver tissue indicators were investigated，and pathological sections of liver tissue were observed.The anti-inebriation and hepatoprotective effects of RZOL were assessed.Additionally，an AML12 liver cell injury model was established with ethanol，and the impact of RZOL on ethanol-stimulated AML12 cell supernatants was measured by examining glutamic-pyruvic transaminase(GPT)，glutamic oxalacetic transaminase(GOT)，and reactive oxygen species(ROS) levels.Results:Mice treated with RZOL had significantly extended intoxication times，shortened sleep time and time to wake-up，significantly reduced serum ethanol concentration，GPT，and GOT levels，improved liver cell damage，increased superoxide dismutase(SOD) activity and glutathione peroxidase(GSH) levels in liver tissue，and reduced malondialdehyde(MDA) content.In AML12 liver cells cultured in vitro，RZOL reversed ethanol-induced increases in ALT and AST activities and excessive ROS production.Conclusion:RZOL demonstrates effective anti-inebriation，detoxification，and liver protection properties.Its mechanism likely involves enhancing ethanol metabolism and boosting endogenous antioxidant enzyme activity. |
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