| 引用本文:林炜凯1，黎芷鑫1，李季2，吴立友2，周艳利2，毛凯凤2.加味芪黄饮对膜性肾病大鼠肾组织细胞凋亡的影响[J].世界中医药,2024,(19):. |
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| 加味芪黄饮对膜性肾病大鼠肾组织细胞凋亡的影响 |
| Effect of Modified Qihuang Decoction on Apoptosis of Renal Tissue in Rats with Membranous Nephropathy |
| 投稿时间：2024-01-23 |
| DOI：10.3969/j.issn.1673-7202.2024.19.006 |
| 中文关键词: 加味芪黄饮 膜性肾病 足细胞 肾功能 细胞凋亡 B细胞淋巴瘤-2 Bcl-2相关X蛋白 肾母细胞瘤基因1 |
| English Keywords:Modified Qihuang Decoction Membranous nephropathy Podocyte Renal function Apoptosis Bcl-2 Bax WT-1 |
| 基金项目:国家自然科学基金项目（81573850）；广东省中医药管理局项目（20210097）；广州市中医药和中西医结合科技项目（2021A011008）；广州医科大学附属中医医院青苗计划（20210128） |
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| 中文摘要: |
| 目的：探究加味芪黄饮对膜性肾病（MN）大鼠肾功能和肾组织细胞凋亡的影响。方法：通过改良Border制备MN大鼠模型，随机分为5组并连续给药4周：空白组（20只）和模型组（15只）灌胃给予生理盐水5 mL；贝那普利组(18只)灌胃给予盐酸贝那普利10 mg/kg；加味芪黄饮低、高剂量组(各18只)分别灌胃给予加味芪黄饮4 mg/kg、8 mg/kg。各组均分别于造模后第2到6周末检测大鼠24 h尿蛋白。造模后第6周末，采用苏木精-伊红（HE）染色观察肾脏病理改变；采用免疫组织化学法检测肾组织B细胞淋巴瘤2相关X蛋白（Bax）、B细胞淋巴瘤2（Bcl-2）及肾母细胞瘤抑制基因1（WT-1）的表达水平，腹主动脉取血检测血尿素氮（BUN）、血肌酐(Scr)、人血白蛋白（Alb）、三酰甘油(TG)、总胆固醇(TC)。结果：模型组较空白组24 h尿蛋白、BUN、Scr、TG、TC、Bax蛋白表达水平升高，Bcl-2、WT-1蛋白表达水平、Alb降低（P<0.01)；各给药组较模型组24 h尿蛋白、BUN、Scr、TG、TC以及Bax蛋白表达水平均降低，Bcl-2、WT-1蛋白表达水平以及Alb均升高（P<0.05，P<0.01)；且加味芪黄饮高剂量组较低剂量组变化更显著。结论：加味芪黄饮可能通过降低Bax蛋白表达水平，提高Bcl-2、WT-1蛋白表达水平，影响细胞凋亡，保护肾脏足细胞，从而延缓膜性肾病大鼠的肾功能损伤。 |
| English Summary: |
| To explore the effect of modified Qihuang Decoction on renal function and renal tissue cell apoptosis in rats with membranous nephropathy(MN).Methods:The MN rat model was established by an improved Border method，and rats were randomly divided into five groups and treated for 4 consecutive weeks.The blank group(20 rats) and the model group(15 rats) were administered 5 mL of physiological saline by gavage.The Benazepril group(18 rats) was administered benazepril hydrochloride at 10 mg/kg by gavage.The low-dose and high-dose modified Qihuang Decoction groups(18 rats each) were administered modified Qihuang Decoction at 4 mg/kg and 8 mg/kg by gavage，respectively.Urine protein levels were measured at the end of weeks 2 to 6.At the end of week 6，renal pathological changes were observed by hematoxylin-eosin(HE) staining.The expression levels of Bcl-2-associated X protein(Bax)，B-cell lymphoma 2(Bcl-2)，and Wilms' tumor suppressor gene 1(WT-1) in renal tissue were detected by immunohistochemistry.Blood samples were collected from the abdominal aorta to measure blood urea nitrogen(BUN)，serum creatinine(Scr)，human serum albumin(Alb)，triglycerides(TG)，and total cholesterol(TC).Results:In the model group，24-hour urine protein，BUN，Scr，TG，TC，and Bax protein expression levels were significantly higher，while Bcl-2，WT-1，and Alb expression levels were significantly lower compared with the blank group(P<0.01).In each treatment group，24-hour urine protein，BUN，Scr，TG，TC，and Bax protein expression levels were significantly lower，while Bcl-2，WT-1，and Alb expression levels were significantly higher compared with the model group(P<0.05，P<0.01).Furthermore，the high-dose modified Qihuang Decoction group showed more significant changes than the low-dose group.Conclusion:Modified Qihuang Decoction may protect podocytes in MN rats by reducing Bax protein expression，increasing Bcl-2 and WT-1 protein expression，and influencing cell apoptosis，thereby delaying renal function impairment in MN. |
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