| 引用本文:周欢1，2，刘亚尊1，2，杨艳1，胡逸中1，韩兆鹏3，薛征1.苦杏仁苷对哮喘模型小鼠肺组织病理及辅助性T细胞17/调节性T细胞免疫平衡的影响[J].世界中医药,2024,(19):. |
|
| 苦杏仁苷对哮喘模型小鼠肺组织病理及辅助性T细胞17/调节性T细胞免疫平衡的影响 |
| Effect of Amygdalin on Pulmonary Histopathology and Th17/Treg Immune Balance in Asthmatic Mice |
| 投稿时间：2021-07-13 |
| DOI：10.3969/j.issn.1673-7202.2024.19.007 |
| 中文关键词: 支气管哮喘 苦杏仁苷 气道炎症 免疫失衡 肺组织病理 白细胞介素-17 白细胞介素-10 |
| English Keywords:Bronchial asthma Amygdalin Airway inflammation Immune imbalance Pulmonary histopathology Interleukin-17 Interleukin-10 |
| 基金项目:国家自然科学基金面上项目(82074488) |
|
| 摘要点击次数: 100 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：通过观察苦杏仁苷对哮喘模型小鼠肺组织病理及辅助性T细胞17（Th17）/调节性T细胞（Treg）免疫平衡的影响，探讨苦杏仁苷干预哮喘模型小鼠气道炎症的作用机制。方法：无特定病原体（SPF）级BALB/c小鼠50只，随机分为空白对照组、模型组、地塞米松组、苦杏仁苷低剂量组和苦杏仁苷高剂量组。运用卵白蛋白（OVA）和氢氧化铝对BALB/c小鼠腹腔注射致敏、雾化吸入激发，建立小鼠支气管哮喘模型。末次激发后给药7 d取材。采用苏木精-伊红（HE）染色法观察小鼠肺组织病理改变，酶联免疫吸附试验法（ELISA）检测左肺支气管肺泡灌洗液（BALF）中炎症介质白细胞介素（IL-10）、IL-17水平。结果：各肺组织病理结果显示，空白对照组细支气管管壁结构正常，管周无明显炎症细胞浸润；模型组细支气管管壁明显增厚，管腔内及周围组织炎症细胞浸润明显；地塞米松组、高剂量组及低剂量组管周组织均伴有不同程度的炎症细胞浸润，支气管管壁增厚不明显。ELISA结果显示，模型组IL-10明显低于空白对照组、而IL-17水平明显高于空白对照组（P<0.01），与模型组比较，各用药组IL-10水平均增高、IL-17水平均降低（P<0.01），与地塞米松组比较，苦杏仁苷低剂量组、苦杏仁苷高剂量组IL-10、IL-17均升高(P<0.01）。结论：不同剂量的苦杏仁苷均可以减轻支气管哮喘模型小鼠肺组织炎症，减少IL-17的分泌，且提高IL-10炎症介质水平的作用优于地塞米松组。 |
| English Summary: |
| To investigate the mechanism of action of amygdalin in modulating airway inflammation in an asthma model by observing its effect on pulmonary histopathology and the Helper T helper 17 cell(Th17)/regulatory T cell(Treg) immune balance in asthmatic mice.Methods:Fifty SPF-grade BALB/c mice were randomly divided into five groups：blank control group，model group，dexamethasone group，low-dose amygdalin group，and high-dose amygdalin group.A mouse model of bronchial asthma was established by sensitizing BALB/c mice via intraperitoneal injection of ovalbumin(OVA) and aluminum hydroxide，followed by aerosolized inhalation.After the final challenge，treatment was administered for 7 days before tissue collection.Lung tissue pathology was observed using hematoxylin-eosin(HE) staining.Levels of inflammatory mediators，interleukin-10(IL-10) and IL-17，in the left lung bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay(ELISA).Results:Pathological examination of the lung tissue showed that the blank control group had normal bronchiolar walls with no significant inflammatory cell infiltration around the bronchioles.In the model group，bronchiolar walls were significantly thickened with marked infiltration of inflammatory cells in the lumen and surrounding tissues.The dexamethasone，high-dose，and low-dose amygdalin groups showed varying degrees of inflammatory cell infiltration around the bronchioles，with less noticeable thickening of the bronchial walls.ELISA results showed that the IL-10 level in the model group was significantly lower than in the blank control group，and the IL-17 level was significantly higher(P<0.01).Compared to the model group，all treatment groups showed an increase in IL-10 and a decrease in IL-17 levels(P<0.01).Both the low-dose and high-dose amygdalin groups exhibited higher IL-10 and IL-17 levels compared to the dexamethasone group(P<0.01).Conclusion:Both low and high doses of amygdalin can reduce lung tissue inflammation in the bronchial asthma model，decrease IL-17 secretion，and increase IL-10 levels，with effects superior to those of dexamethasone. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
