| 引用本文:李丹1，胡凯文2，韩丽3，赵百孝4.艾灸对癌性疲劳小鼠磷脂酰肌醇3激酶-蛋白激酶B信号通路的影响[J].世界中医药,2024,(19):. |
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| 艾灸对癌性疲劳小鼠磷脂酰肌醇3激酶-蛋白激酶B信号通路的影响 |
| Effects of Moxibustion on PI3K-AKT Signaling Pathway in Cancer-related Fatigue Mice |
| 投稿时间：2022-04-11 |
| DOI：10.3969/j.issn.1673-7202.2024.19.008 |
| 中文关键词: 艾灸 红外灸 癌性疲劳 炎症介质 PI3K-AKT信号通路 荷瘤动物 顺铂 |
| English Keywords:Moxibustion Infrared moxibustion Cancer-related fatigue Inflammatory mediator PI3K-AKT signaling pathway Tumor-bearing animals Cisplatin |
| 基金项目:国家重点研发计划项目（2019YFC1711904） |
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| 中文摘要: |
| 目的：观察艾灸对癌性疲劳（CRF）小鼠及磷脂酰肌醇3激酶-蛋白激酶B（PI3K-AKT）信号通路的影响。方法：将BALB/C小鼠随机分为空白组10只、模型组15只、艾灸组15只、红外组15只。空白组不进行任何处理，模型组选择4T1乳腺癌细胞系皮下移植联合顺铂构建CRF动物模型，艾灸组及红外组分别予艾灸、红外照射CRF小鼠关元穴4周。干预治疗后检测小鼠行为学（疲劳跑台实验、悬尾实验），并运用酶联免疫吸附试验检测血清白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）含量，蛋白质免疫印迹法检测肿瘤组织磷酸化磷脂酰肌醇3激酶（P-PI3K)、蛋白激酶B（AKT）、磷酸化蛋白激酶B(P-AKT)、B淋巴细胞瘤-2基因（Bcl-2）、胱天蛋白酶-3（Caspase-3）蛋白表达，RT-PCR法检测肿瘤组织IL-6 mRNA及PI3K mRNA表达。结果：与空白组比较，模型组小鼠疲劳跑台时间及距离减少，脾脏指数、跑台电击次数及悬尾时间，血清IL-6、TNF-α含量增加；与模型组比较，干预小鼠跑台时间及距离增加，跑台电击次数和悬尾不动时间，瘤重、脾脏指数、IL-6、TNF-α、P-PI3K、P-AKT与IL-6 mRNA、PI3K mRNA减少，艾灸组Bcl-2含量减少，Caspase-3表达增加；与红外组比较，艾灸组小鼠悬尾不动时间、IL-6、TNF-α、P-PI3K、IL-6mRNA表达减少。结论：艾灸可改善CRF，其机制可能是通过炎症介质介导的PI3K-AKT信号通路发挥作用，而红外线是艾灸起效的重要因素。 |
| English Summary: |
| To observe the effects of moxibustion on cancer-related fatigue(CRF) in mice and its impact on the phosphoinositide 3-kinase(PI3K)-protein kinase B(AKT) signaling pathway.Methods:BALB/C mice were randomly divided into four groups：blank control group(n=10)，model group(n=15)，moxibustion group(n=15)，and infrared group(n=15).The blank control group received no treatment.The model group was induced with a CRF animal model by subcutaneously transplanting 4T1 breast cancer cells and administering cisplatin.The moxibustion and infrared groups received moxibustion and infrared radiation at the Guanyuan(BL 26) for 4 weeks，respectively.After the intervention，the behavior of the mice was assessed through fatigue treadmill experiment and tail suspension test.Serum levels of interleukin-6(IL-6) and tumor necrosis factor-alpha(TNF-α) were measured using enzyme-linked immunosorbent assay(ELISA).Western blot was used to detect protein expression of phosphorylated PI3K(p-PI3K)，AKT，phosphorylated AKT(p-AKT)，B-cell lymphoma 2(Bcl-2)，and caspase-3 in tumor tissues.Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of IL-6 and PI3K in tumor tissues.Results:Compared with the blank control group，the model group exhibited a reduction in fatigue treadmill time and distance，an increase in spleen index，treadmill shock frequency，and tail suspension time，as well as elevated serum levels of IL-6 and TNF-α.Compared with the model group，the intervention groups showed an increase in treadmill time and distance，a reduction in shock frequency and tail immobility time，a decrease in tumor weight，spleen index，IL-6，TNF-α，p-PI3K，p-AKT，IL-6 mRNA，and PI3K mRNA levels.In the moxibustion group，Bcl-2 levels were reduced，while caspase-3 expression was increased.Compared with the infrared group，the moxibustion group had reduced tail immobility time，IL-6，TNF-α，p-PI3K，and IL-6 mRNA expression.Conclusion:Moxibustion can alleviate CRF，and its mechanism may involve the PI3K-AKT signaling pathway mediated by inflammatory mediators.Infrared radiation plays a significant role in the effectiveness of moxibustion. |
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