| 引用本文:邱咏薇1，闵彤彤1，周鹏1，张亚梅1，杨书洁2，钟国跃1，3.藏药塞北紫堇总生物碱降尿酸活性及其化学成分研究[J].世界中医药,2024,(24):. |
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| 藏药塞北紫堇总生物碱降尿酸活性及其化学成分研究 |
| Uric Acid-lowering Activity and Chemical Constituents of Total Alkaloids from Corydalis impatiens |
| 投稿时间：2024-02-27 |
| DOI：10.3969/j.issn.1673-7202.2024.24.002 |
| 中文关键词: 塞北紫堇 生物碱 高尿酸血症 藏药 代谢性疾病 哇夏嘎 活性成分 |
| English Keywords:Corydalis impatiens Total alkaloids Hyperuricemia Tibetan medicine Metabolic disease Wasaga Active constituents |
| 基金项目:国家重点研发计划项目（2019YFC1712300）——藏、蒙、维等民族药资源信息化共享平台构建、品种整理及繁育保护技术研究；江西省教育厅科学技术研究项目（GJJ211229）——基于JNK/p38信号通路探讨藏药玉冬赛果抗心肌缺血活性成分及作用机制 |
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| 中文摘要: |
| 目的：探讨藏药塞北紫堇总生物碱的降尿酸活性及可能的作用机制，并对其化学成分进行分离鉴定。方法：将塞北紫堇总生物碱灌胃由氧嗪酸钾盐诱导的高尿酸血症模型小鼠7 d，用试剂盒测定血清尿酸（UA）、尿素氮（BUN）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）水平，肝脏黄嘌呤氧化酶（XOD）和腺苷脱氨酶（ADA）活力。结果：与模型组比较，塞北紫堇总生物碱高、低剂量均能够显著降低急性高尿酸血症模型小鼠的血清UA、BUN和TNF-α含量（均P<0.01），显著抑制肝脏XOD和ADA活力（P<0.01，P<0.05），且呈现剂量依赖性；进一步从塞北紫堇总生物碱中分离得到11个异喹啉类生物碱成分，即吡咕咕灵（1）、四氢黄连碱（2）、碎叶紫堇碱（3）、卡维丁（4）、北美黄连碱（5）、去氢碎叶紫堇碱（6）、氧化北美黄连次碱（7）、白屈菜碱（8）、紫堇明碱（9）、紫堇块茎碱（10）、原阿片碱（11）。结论：塞北紫堇总生物碱具有显著降尿酸和肾脏保护作用，异喹啉类生物碱是其主要活性成分，且其可能的作用机制包括抑制XOD和ADA活力，以及下调炎症介质TNF-α表达等；此外，化合物6、8为首次从塞北紫堇中分离得到。 |
| English Summary: |
| To investigate the uric acid-lowering activity and possible mechanisms of action of the total alkaloids from Corydalis impatiens，and to isolate and identify its chemical components.Methods:The total alkaloids from C.impatiens were administered by gavage to mice with hyperuricemia induced by potassium oxonate for 7 days.Serum uric acid(UA)，blood urea nitrogen(BUN)，tumor necrosis factor-α(TNF-α)，and interleukin-1β(IL-1β) levels were measured using assay kits.The activities of hepatic xanthine oxidase(XOD) and adenosine deaminase(ADA) were also assessed.Results:Compared with the model group，both high and low doses of the total alkaloids of C.impatiens significantly reduced serum UA，BUN，and TNF-α levels(all P<0.01)，and significantly inhibited the activities of hepatic XOD and ADA(P<0.01，P<0.05)，showing a dose-dependent effect.Eleven isoquinoline alkaloids were isolated from C.impatiens total alkaloids，including bicuculline(1)，tetrahydrocoptisine(2)，cheilanthifoline(3)，corydaine(4)，β-hydrastine(5)，dehydrocheilanthifoline(6)，oxyhydrastinine(7)，chelidonine(8)，corlumine(9)，corytuberine(10)，and protopine(11).Conclusion:The total alkaloids from C.impatiens have significant uric acid-lowering and kidney-protective effects.Isoquinoline alkaloids are the main active components，and the potential mechanisms include inhibiting XOD and ADA activities and downregulating the expression of inflammatory mediators such as TNF-α.Additionally，compounds 6 and 8 were isolated for the first time from C.impatiens. |
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