| 引用本文:韩福国1，2，王磊3，周景春3，冯兴中4，范雪梅1，2，郝艳丽1，2，刘清飞1，2.基于成分与微粒体系探讨冷冻干燥对中药汤剂质量与吸收的影响[J].世界中医药,2025,(03):. |
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| 基于成分与微粒体系探讨冷冻干燥对中药汤剂质量与吸收的影响 |
| Effect of Freeze-drying on the Quality and Oral Absorption of Chinese Medicine Decoctions Based on the Composition and Microparticle Dispersion System |
| 投稿时间：2024-05-11 |
| DOI：10.3969/j.issn.1673-7202.2025.03.003 |
| 中文关键词: 中药汤剂 冷冻干燥 指纹图谱 质量评价 相似度评价 化学成分 自组装聚集体 超高效液相色谱-质谱 |
| English Keywords:Chinese medicine decoction Freeze-drying Fingerprint Quality evaluation Similarity evaluation Chemical composition Self-assembled aggregates UHPLC-MS |
| 基金项目:国家科技重大专项（2023ZD0506606） |
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| 摘要点击次数: 9 |
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| 中文摘要: |
| 目的：从化学成分与微粒分散体系角度，探讨冷冻干燥对中药汤剂的质量与口服吸收的影响。方法：以4种中药复方汤剂（其中2种含芳香类药材）为研究对象，基于超高效液相色谱（UHPLC）法和气相色谱（GC）法比较冻干前后样品的指纹图谱的相似度，同时检测冻干前后样品的醇沉沉淀量、沉淀物中多糖与蛋白含量、自组装聚集体的粒径与Zeta电位分布，从多角度评价冷冻干燥对中药汤剂质量的影响。采用超高效液相色谱-质谱（UHPLC-MS）法，鉴定大鼠灌胃给药的入血成分，评价冷冻干燥对中药汤剂口服吸收的影响。结果：4种中药复方汤剂冻干前后样品的UHPLC指纹图谱相似度均较高。中药复方汤剂冻干前后醇沉沉淀量、沉淀物中多糖与蛋白质的含量，以及粒径与Zeta电位，总体上差异不大。含有芳香类药材的复方汤剂，冻干前后GC指纹图谱之间的相似度较低，且冻干样品中挥发性成分的含量均有显著性降低（P<0.05）。对大鼠含药血浆检测发现，冷冻干燥对汤剂吸收入血的化学成分的数量和浓度无显著影响（P>0.05）。结论：对于不含芳香类药材的中药复方，冷冻干燥对汤剂的成分、微粒体系，以及成分吸收无显著影响，可保证临床药效。对于含有芳香类药材的中药复方，冷冻干燥会导致挥发性成分的损失，利用冷冻干燥技术时必须慎重考虑。 |
| English Summary: |
| To investigate the effect of freeze-drying on the quality and oral absorption of Chinese medicine decoctions from the perspectives of chemical composition and microparticle dispersion system.Methods:Four Chinese medicine compound decoctions，including two containing aromatic medicinal herbs，were selected for study.Ultra-high-performance liquid chromatography(UHPLC) and gas chromatography(GC) were used to compare the fingerprint similarity of samples before and after freeze-drying.Additionally，the ethanol precipitation yield，polysaccharide and protein content in the precipitate，particle size，and Zeta potential distribution of self-assembled aggregates were measured to evaluate the effect of freeze-drying on Chinese medicine decoction quality.Ultra-high-performance liquid chromatography-mass spectrometry(UHPLC-MS) was employed to identify blood-absorbed components in rats after oral administration，assessing the influence of freeze-drying on oral absorption.Results:The UHPLC fingerprints of the four Chinese medicine compound decoctions before and after freeze-drying showed high similarity.No significant differences were observed in ethanol precipitation yield，polysaccharide and protein content in the precipitate，particle size，or Zeta potential distribution between pre-and post-freeze-dried samples.However，for decoctions containing aromatic medicinal herbs，GC fingerprint similarity was lower after freeze-drying，with a significant reduction in volatile components(P<0.05).Analysis of medicated rat plasma indicated no significant changes in the number or concentration of blood-absorbed chemical components due to freeze-drying(P>0.05).Conclusion:For non-aromatic Chinese medicine compound decoctions，freeze-drying does not significantly affect composition，microparticle dispersion system，or component absorption，ensuring clinical efficacy.However，for Chinese medicine compound decoctions containing aromatic medicinal herbs，freeze-drying leads to the loss of volatile components，necessitating careful consideration when applying this technique. |
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