| 引用本文:刘浩1，骆帝2，阎伟2，李金松2，闫德志1.补肾活血汤对地塞米松处理后大鼠骨髓间充质干细胞的影响[J].世界中医药,2025,(03):. |
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| 补肾活血汤对地塞米松处理后大鼠骨髓间充质干细胞的影响 |
| Effect of Bushen Huoxue Decoction on Bone Marrow Mesenchymal Stem Cells in Rats Treated with Dexamethasone |
| 投稿时间：2024-04-29 |
| DOI：10.3969/j.issn.1673-7202.2025.03.008 |
| 中文关键词: 补肾活血汤 含药血清 骨髓间充质干细胞 地塞米松 刺猬信号通路 细胞增殖 细胞迁移 细胞成骨分化 |
| English Keywords:Bushen Huoxue Decoction Drug-medicated serum Bone marrow mesenchymal stem cells(BMSCs) Dexamethasone Hedgehog signaling pathway Cell proliferation Cell migration Cell osteogenic differentiation |
| 基金项目:国家自然科学基金项目（82074453，82205154）；山东省重点研发计划（重大科技创新工程）项目（2021CXGC010501）；山东省自然科学基金青年项目（ZR2021QH004）；山东省自然科学基金联合专项基金项目（ZR2021LZY002）；2020年齐鲁卫生与健康杰青人才培育工程项目（rc2021002-23） |
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| 中文摘要: |
| 目的：基于刺猬（Hh）信号通路探讨补肾活血汤含药血清对地塞米松（Dex）处理后大鼠骨髓间充质干细胞（BMSC）增殖、迁移及成骨分化的影响。方法：贴壁法提取大鼠BMSC，评估纯度和活性。将大鼠BMSC分为8组：对照组、补肾活血汤低、中、高剂量组（0.662、1.323、2.646 g/kg补肾活血汤灌胃大鼠血清）、Dex组、Dex+补肾活血汤低、中、高剂量组（Dex+0.662、1.323、2.646 g/kg补肾活血汤灌胃大鼠血清）。干预48 h后，细胞计数试剂盒-8（CCK-8）法检测BMSC增殖，细胞划痕实验检测BMSC迁移，荧光定量聚合酶链反应（qPCR）检测BMSC中成骨相关指标[碱性磷酸酶（ALP）、Ⅰ型胶原蛋白（COL Ⅰ）、Runt相关转录因子2（RUNX2）、骨桥蛋白（OPN）]及Hh信号通路因子[音猬因子（Shh）、神经胶质瘤相关癌基因同源蛋白1（Gli1）、Gli2]mRNA表达。结果：贴壁法提取的BMSC具有较高纯度和良好的分化潜能，补肾活血汤含药血清能显著逆转Dex对BMSC增殖、迁移和成骨分化潜能的抑制作用。与Dex组比较，中剂量补肾活血汤含药血清上调BMSC中成骨因子（ALP、COL I、RUNX2）和Hh信号通路因子（Gli1、Gli2、Shh）mRNA和蛋白表达（均P<0.05），中、高剂量补肾活血汤含药血清上调OPN蛋白表达（均P<0.05）。结论：补肾活血汤含药血清能通过上调Hh信号通路因子，促进Dex处理后BMSC增殖、迁移及成骨分化。 |
| English Summary: |
| To investigate the effect of Bushen Huoxue Decoction(BHD)-medicated serum on the proliferation，migration，and osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs) treated with dexamethasone(Dex) via the Hedgehog(Hh) signaling pathway.Methods:BMSCs were isolated from rats using the adherent culture method，and their purity and viability were assessed.The BMSCs were divided into eight groups：control，low-，medium-，and high-dose BHD groups(serum from rats receiving 0.662，1.323，and 2.646 g/kg BHD，respectively)，Dex group，and Dex+low-，medium-，and high-dose BHD groups(Dex+serum from rats receiving 0.662，1.323，and 2.646 g/kg BHD，respectively).After 48 hours of intervention，cell proliferation was assessed using the Cell Counting Kit-8(CCK-8)，and migration was evaluated using a scratch assay.The mRNA expression of osteogenic differentiation markers [alkaline phosphatase(ALP)，type I collagen(COL I)，Runt-related transcription factor 2(RUNX2)，and osteopontin(OPN)] and Hh signaling pathway factors [Sonic hedgehog(Shh)，glioma-associated oncogene homolog 1(Gli1)，and Gli2] was analyzed using quantitative real-time PCR(qPCR).Results:BMSCs isolated by the adherent culture method exhibited high purity and good differentiation potential.BHD-medicated serum significantly reversed the inhibitory effects of Dex on BMSC proliferation，migration，and osteogenic differentiation.Compared with the Dex group，the medium-dose BHD-medicated serum significantly upregulated the mRNA and protein expression of osteogenic markers(ALP，COL I，RUNX2) and Hh signaling pathway factors(Shh，Gli1，Gli2) in BMSCs(all P<0.05).Moreover，both medium-and high-dose BHD-medicated serum significantly increased OPN protein expression(both P<0.05).Conclusion:BHD-medicated serum promotes the proliferation，migration，and osteogenic differentiation of Dex-treated BMSCs by upregulating Hh signaling pathway factors. |
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