| 引用本文:刘海燕1，付明月2，孙海珍3，曾玉英1.汉黄芩素对乙型肝炎病毒大鼠肝功能的影响[J].世界中医药,2025,(03):. |
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| 汉黄芩素对乙型肝炎病毒大鼠肝功能的影响 |
| Effect of Wogonin on Liver Function of Rats with Hepatitis B Virus |
| 投稿时间：2024-05-11 |
| DOI：10.3969/j.issn.1673-7202.2025.03.009 |
| 中文关键词: 汉黄芩素 乙型肝炎病毒 磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路 肝功能 大鼠模型 病理损伤 纤维化 感染 |
| English Keywords:Wogonin Hepatitis B virus Phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway Liver function Rat model Pathological injury Fibrosis Infection |
| 基金项目:国家自然科学基金项目（31560292） |
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| 中文摘要: |
| 目的：探讨汉黄芩素对乙型肝炎病毒（HBV）大鼠肝功能的影响及其作用机制。方法：斯泼累格·多雷（Sprague-Dawley，SD）雄性大鼠72只，随机分为对照组，HBV模型组，IFN-α组，汉黄芩素低、中、高剂量组，每组12只。除对照组，其余大鼠采用尾静脉注射HBV抗原构建HBV大鼠模型。IFN-α组大鼠高压尾静脉注射10 μg pKCMvint.IFN-α，汉黄芩素低、中、高剂量组大鼠分别腹腔注射汉黄芩素10 mg/kg、20 mg/kg、40 mg/kg，对照组和模型组大鼠腹腔注射生理盐水10 mL/(kg·d)，持续2周。2周后处死，酶联免疫吸附试验（ELISA）法测定大鼠血清HBV标志物乙型肝炎e抗原（HBeAg）、乙型肝炎表面抗原（HBsAg）和肝功能指标谷丙转氨酶（GPT）、谷草转氨酶（GOT）水平，苏木精-伊红（HE）染色和马松（Masson）染色分别检测肝组织病理学改变和纤维化情况，蛋白免疫印迹（Western blotting）检测肝组织匀浆中磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）/哺乳动物雷帕霉素靶蛋白（mTOR）通路蛋白表达。结果：与HBV模型组比较，汉黄芩素中、高剂量组大鼠饮食和排泄基本正常，腹腔积液消失，肝组织损伤缓解，肝纤维化减少，血清HBeAg、HBsAg、GOT、GPT水平和肝组织p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR蛋白水平显著降低（均P<0.05）。结论：汉黄芩素可保护HBV大鼠肝功能，其可能的机制是抑制PI3K/AKT/mTOR信号通路。 |
| English Summary: |
| To investigate the effects and underlying mechanisms of wogonin on liver function in hepatitis B virus(HBV) model rats.Methods:Seventy-two male Sprague-Dawley(SD) rats were randomly divided into six groups(n=12 per group)：control，HBV model，IFN-α，and low-，medium-，and high-dose wogonin groups.Except for the control group，all rats were injected with HBV antigen via the tail vein to establish the HBV model.The IFN-α group received a high-pressure tail vein injection of 10 μg of pKCMvint.IFN-α，while the wogonin groups received intraperitoneal injections of 10 mg/kg，20 mg/kg，or 40 mg/kg wogonin，respectively.The control and model groups received normal saline(10 mL/kg·d) intraperitoneally for two weeks.After two weeks，the rats were sacrificed，and serum HBV markers，including hepatitis B e-antigen(HBeAg) and hepatitis B surface antigen(HBsAg)，as well as liver function indicators alanine aminotransferase(GPT) and aspartate aminotransferase(GOT)，were measured using enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE) and Masson staining were used to assess liver histopathological changes and fibrosis.Western blot was performed to analyze the expression of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR) signaling pathway proteins in liver tissue homogenates.Results:Compared with the HBV model group，the medium-and high-dose wogonin groups showed improved dietary intake and excretion，disappearance of ascites，alleviation of liver tissue damage，and reduced liver fibrosis.Serum levels of HBeAg，HBsAg，GOT，and GPT，as well as liver tissue p-PI3K/PI3K，p-AKT/AKT，and p-mTOR/mTOR protein levels，were significantly decreased(P<0.05).Conclusion:Wogonin protects liver function in HBV model rats，potentially through inhibition of the PI3K/AKT/mTOR signaling pathway. |
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