| 引用本文:贾璐，肖琪，张仲瑾，王青，李彦青，樊慧杰，柴智，马存根.五味子乙素对帕金森病小鼠神经炎症的影响[J].世界中医药,2025,(03):. |
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| 五味子乙素对帕金森病小鼠神经炎症的影响 |
| Effect of Schisandrin B on Neuroinflammation in PD Mice |
| 投稿时间：2024-05-24 |
| DOI：10.3969/j.issn.1673-7202.2025.03.010 |
| 中文关键词: 五味子乙素 帕金森病 MPTP 神经炎症 NOD样受体蛋白3 凋亡相关斑点样蛋白 剪切的胱天蛋白酶-1 N端-Gasdermin D蛋白 |
| English Keywords:Schisandrin B Parkinson's disease MPTP Neuroinflammation NLRP3 ASC Cleaved-caspase1 GSDMD-N |
| 基金项目:国家中医药管理局青年岐黄学者培养项目（国中医药人教函〔2022〕256号）；山西省自然科学基金项目（202203021212341）；山西中医药大学博士科研启动基金项目（2020BK17）；山西中医药大学科技创新能力培育计划项目（2020PY-JC〔Y〕03） |
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| 中文摘要: |
| 目的：探讨五味子乙素（Sch B）对帕金森病（PD）模型小鼠NOD样受体蛋白3（NLRP3）信号通路的影响。方法：将30只C57BL/6雄性小鼠随机分为正常组、模型组和Sch B组，每组10只。模型组和Sch B组采用腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）建立PD模型，正常组注射等量生理盐水。除正常组，Sch B组小鼠从造模第1天给予80 mg/(kg·d) Sch B灌胃，模型组和正常组小鼠予以等体积0.5%羧甲基纤维素钠灌胃，连续干预14 d。采用爬杆实验观察小鼠行为学表现，采用免疫荧光染色法检测小鼠黑质区离子钙接头蛋白（Iba-1）表达，采用蛋白质免疫印迹法检测黑质区CD11b、白细胞介素18（IL-18）、白细胞介素1β（IL-1β）、NLRP3、凋亡相关斑点样蛋白（ASC）、剪切的胱天蛋白酶-1（cleaved-Caspase 1）和N端-Gasdermin D蛋白（GSDMD-N）表达。结果：与正常组比较，模型组小鼠爬杆时间明显延长(P<0.01)，Iba-1荧光强度显著增强(P<0.01)，CD11b、IL-18、IL-1β、NLRP3、ASC、cleaved-Caspase 1和GSDMD-N蛋白表达明显增加(P<0.05或P<0.01)。与模型组比较，Sch B组小鼠爬杆时间显著减少，Iba-1荧光强度显著减弱，同时CD11b、IL-18、IL-1β、NLRP3、ASC、cleaved-Caspase1和GSDMD-N蛋白表达显著减少(P<0.05或P<0.01)。结论：Sch B能够减轻MPTP诱导的PD小鼠神经炎症，这可能与干预NLRP3信号通路有关。 |
| English Summary: |
| To investigate the effect of Schisandrin B(Sch B) on the NOD-like receptor protein 3(NLRP3) signaling pathway in a Parkinson's disease(PD) mouse model.Methods:Thirty male C57BL/6 mice were randomly divided into a normal group，a model group，and a Sch B group，with 10 mice in each group.The PD model was established in the model and Sch B groups via intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)，while the normal group received an equivalent volume of normal saline.From the first day of modeling，mice in the Sch B group were administered Sch B(80 mg/kg·d) via gavage，whereas the model and normal groups received an equivalent volume of 0.5% sodium carboxymethylcellulose by gavage for 14 consecutive days.The pole test was used to assess behavioral performance.Immunofluorescence staining was performed to detect the expression of ionized calcium-binding adapter molecule 1(Iba-1) in the substantia nigra.Western blot analysis was conducted to measure the expression of CD11b，interleukin-18(IL-18)，interleukin-1β(IL-1β)，NLRP3，apoptosis-associated speck-like protein(ASC)，cleaved caspase-1，and N-terminal gasdermin D(GSDMD-N) in the substantia nigra.Results:Compared with the normal group，the model group exhibited a significantly prolonged pole test time(P<0.01)，increased Iba-1 fluorescence intensity(P<0.01)，and upregulated expression of CD11b，IL-18，IL-1β，NLRP3，ASC，cleaved caspase-1，and GSDMD-N(P<0.05 or P<0.01).Compared with the model group，the Sch B group showed a significant reduction in pole test time and Iba-1 fluorescence intensity，along with decreased expression of CD11b，IL-18，IL-1β，NLRP3，ASC，cleaved caspase-1，and GSDMD-N(P<0.05 or P<0.01).Conclusion:Sch B can alleviate neuroinflammation in MPTP-induced PD mice，possibly by modulating the NLRP3 signaling pathway. |
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