To establish a congenital hepatitis B model with ducks, and select effective anti-HBV drugs. Methods: A survey on natural infection rate was carried out among Anas Platyrhynchos Linnaeus in the area of Guang Zhou using Dot Blot, then a comparison of DHBV-DNA level was drawn between congenital and after -birth infected ducks. Anti-virus effect of several chemicals and Chinese material medical were also evaluated using the established model. Results:Hatching farm A had the highest natural infection rate at 46.7%, congenitally infected ducks had more stable and lasting level of DHBV-DNA than those infected after birth. Nucleoside drugs could inhibit that level to various extents upon medication; nevertheless, symptoms rebounded on withdrawal of drugs which resembled clinical treatment of hepatitis B. Extracts of Phyllanthus urinaria L showed good anti-virus effects. Conclusions: Congenitally infected Anas Platyrhynchos Linnaeus are ideal model for hepatitis B study.