To explore preventive and treatment effects of Gu Bi Xiao on joint injuries and its mechanism, providing lab data for Ankylosing Spondylitis treatment.Methods:Forty Wister male rats were randomly divided into 4 groups (n=10): the model group, Chinese medicine group, Rofecoxib group, the three of which established AIA model by injecting Freud' s Adjuvant into the plantar surface of left hind paw, and control group injected with same volume of Sodium Chloride. Chinese medicine decoction (2mL/200g) and Rofecoxib (2mL/200g), or tap water (2mL/200g) were administered respectively to four groups by gavages daily.Results:TNF-а, IL-1β, IL-6 in Rats with AIA decreased markedly after administration of Gu Bi Xiao. Conclusion:Gu Bi Xiao is effective in the treatment of AIA by relieving inflammation and joint swelling and improving joint functions, which may have something to do with down-regulation of TNF-а, IL-1β, IL-6.