To study the therapeutic effects and mechanism of Hu Zhi Mixture (HZM) on rat model of acute liver injure induced by α-Naphthyl iso-thiocyanate (ANIT).Methods:The rats were randomly divided into four groups：normal group, ANIT group, HZM group and Prednison group. The rat model was established by ANIT. 48hours later, we determined the levels of tumor factor-α(TNF-α), malondialdehyde(MDA),superoxide disnutase (SOD), examined liver functions and liver tissues under the microscope.Results:Compared with ANIT group, the levels of TNF-α、MDA were markedly lowered in HZM group while the level of SOD markedly increased. Besides, HZM can significantly offset the changes in liver tissue and function induced by ANIT. Prednisone can improve the above values to a certain degree.Conclusion:HZM has good preventive and therapeutic effect against acute liver injure of rats which may be linked with inhibition of TNF-α and MDA, activation of SOD activity.