To observe efficacy of iontophoresis of chinese medicine for ankylosing spondylitis (AS). Methods:Eighty AS patients were randomized to the control group (Meloxicam 7.5mg, Omeprazole 20mg, Sulfasalazine 1.0g, Zhengqing Fengtonning Tablet 60mg, twice per day, Compound Danshen Injection 20mL), and treatment group which were given iontophoresis of lotion No.3 (20min, once per day, 14 days) in addition to abovementioned treatments. Results: 1) Total effective rates were statistically the same (P>0.05), while the treatment group had higher effective rate (P<0.05); 2) While both groups improved significantly in physical signs(P<0.05), the treatment group showed better effect(P<0.01). Conclusion: Lotion No.3 is effective and safe in alleviation of AS symptoms and signs.