To explore the intervention mechanism of Jieyu Yihao, a Chinese compound for promotion of qi in spleen and stomach, on SS' regulating function of HPA axis.Methods:Sixty SD rats, randomly allocated to normal control group, model control group, amitriptyline group, and Jieyu Yihao group, were raised lonely, and given chronic, mild and unforeseeable stress. To measure CRH, SS, CORT by radio-immunity methods, and carry out correlation analysis.Results:SS was negatively correlated to CRH (r=-0.5094，P=0.1972，t=1.4501), and not significantly correlated to CORT(r=0.010，P=0.9613，t=0.0244).Conclusion:By inhibiting the release of CRH, SS regulate HPA axis. Jieyu Yihao can intervene SS' regulating function on HPA axis which may explain its mechanism for anti-depression.