To explore the impact of exogenous estrogens on the neurotransmitters (NT) of rats in order to discover the intervention mechanism of Jingqing Capsule. Methods:The rats were injected subcutaneously with benzestrofol to achieve high-level estrogen. The treatment group was given Jiangqiao Capsules decoction and controlled by a normal group, a model group, and a group treated with Zoloft. Through determining the contents of 5-HT、5-HIAA、NE、DA, discussed was a possible intervention mechanism for depression induced by high-level estrogen. Results:Exogenous estrogen can significantly lower the levels of 5-HT、5-HIAA、NE、DA in rats. Conclusion: Jingqing Capsule can treat or control depression induced by high-level estrogen through raising NT contents.