To discuss the role of endothelin (ET) and nitrogen monoxidum (NO) in gastrointestinal motility dysfunction and the mechanism of catgut embedment at acu-points for early intervention. Methods:In the prospective randomized controlled animal trial, the diabetic model was established on 38 rats by giving lipoproteine rich feeds and intraperitoneal injection of streptozotocin, which were randomized to a positive control group, or be embedded with catgut at acupoints (12 times, once per 7 days). Another 13 healthy rats were taken as normal controls. After 12 week, the contents of ET and NO in sinus ventriculi were determined. Results:The positive control group showed significantly higher ET content, and significantly lower NO content (P<0.05) when compared with the embedment and normal groups, the two of which were not statistically different (P>0.05). Conclusion: The gastrointestinal microcirculation disturbance could be the main pathological basis of the diabetic gastrointestinal compilation, as indicated by the content changes of ET and NO, which can be regulated by acupoint catgut embedment if applied at the early stage.