To investigate impact of Panax Notoginsenosidum and Tamoxifen (TAM) on breast cancer T47D Cell. Methods:Different concentration of TAM and Panax Notoginsenosidum were administered to T47D Cell. The cell growth inhibition was assessed by MTT, the cell cycle distribution and apoptosis rate were assessed by FCM. Results:Panax Notoginsenosidum and Tamoxifen (TAM) can respectively inhibit growth of T47D cell, positively correlated with higher concentration and increasing time. TAM 1.5×10-5mol／L on top of 3 different concentration of Panax Notoginsenosidum had more significant inhibition than used alone. Both can reduce amount of cell at cycle S in comparison with blank control group. Moreover, combined use had the tendency to stimulate apoptosis. Conclusion: Panax Notoginsenosidum has direct, time and concentration dependent inhibition effect on breast cancer T47D Cell. High dose of Panax Notoginsenosidum can enhance anti-tumor effect of TAM by impacting cell cycle and apoptosis.