To investigate impairment mechanism of TNF-α in ischemia-reperfusion rats and effect of Qingkailing. Methods:The ischemia-reperfusion model of rats was established by performing double common carotid clamping on adult male Wistar rats. Qingkailing, antibody-TNF-α, Qingkailing +anti-TNF-α were receptively administered to the model. We observed serum TNF-α、serum sICAM-1、serum p-selectin and expression of brain tissue ICAM-1 at 24h and 72h. Results:A tendency was observed in Qingkailing to reduce the increasing level of serum TNF-α, which intensified over time. Qingkailing and antibody-TNF-α can reduce expression of peripheral blood sICAM-1、P-selectin, and brain tissue ICAM-1. The effect was more obvious after blocking TNF-α, and the effect of Qingkailing was more significant. Conclusion: The BBB injury due to cerebral ischemia-reperfusion is related closely to the toxicity of inflammary cytokine inside and outside the blood capillary, and also related to the adherence between white blood cells and endothelial cells caused by adhesion molecule.