To observe the clinical effectiveness and mechanism of TCM Tonification Method combined with chemotherapy for colon cancer treatment during different stages, and verify an optimized TCM Tonification regimen.Methods:We injected human LoVo cell tumor xenografts into immunodeficiency mice, randomized 60 mice into 6 groups: early intervention group, Simultaneous intervention group, delayed intervention group, chemotherapy group, Chinese herbal medicine group and model group. From the 8th day, the groups received respective treatments. Growth of tumor, food intake, water intake and movements of mice were observed every day. At end of treatment, the expression of Bcl-2 and Bax, and survival time were observed.Results:①average size of tumor: Except the Chinese herbal medicine group, there was significantly reduction of tumor size (P<0.05), and the delayed intervention group and the chemotherapy group were also statistically different (P<0.05). ②average tumor weight: Except the Chinese herbal medicine group and chemotherapy group, the tumor weight in various groups were statistically less than model group (P<0.05), and the delayed intervention group and chemotherapy group had statistically differences (P<0.05).③Tumor inhibition rate: 1.89% in Chinese herbal medicine group, 7.55% in chemotherapy group, 13.21% in simultaneous intervention group, 13.21% in early intervention group,18.87% in delayed intervention group.④The average survival time: Except Chinese herbal medicine group and chemotherapy group, the other groups had significantly longer survival time than that of model group (P<0.05), and there was significant difference between early intervention group and chemotherapy group, between delayed intervention group and chemotherapy group (P<0.05).⑤the average optical density of Bcl-2: There were significantly difference between the early intervention group and model group，between delayed intervention group and model group (P<0.05), and also between delayed intervention group and the chemotherapy group, (P<0.05).⑥The average optical density of Bax: Except Chinese herbal medicine group and chemotherapy group, the other different drugs intervention group and model group were significantly different (P<0.05).Conclusion:Delayed intervention group has a greater survival benefit than early intervention group. The mechanism may be associated with expression of apoptotic protein Bcl-2, Bax .