To investigate modifying impact and mechanism of Ginsenoside saponins (GSS) on bone biomechanics and bone density in glucocorticoid induced osteoporosis rats.Methods:Three month aged female Wister rats were randomly divided into 5 groups, namely the normal control group, model group (hormone treated) and ginseng saponin in high, low, group plus hormone treatment. Subcutaneous injection of methylprednisolone (5mg/Kg) to establish the osteoporosis model. Ginseng saponins groups were given GSS at respective dosages, i.e. high (100mg/kg), medium (50 mg / kg ), low ( 25 mg / kg ), twice a day. The other two groups weregiven the same amount of normal saline. After 9W, rats' bone mineral density (BMD) and biochemical markers of bone metabolism and bone biomechanics were observed.Results:The index of BGP, CT and Ca of the model group were significantly lower than those of the normal group, and of the high dosage GSS group (P<0.05). The difference of RACP and serum P were not significant among groups (P>0.05); Compared with normal group, the rats in the model group showed reduced lumbar and femoral bone mineral density, maximum load and stiffness (P<0.05), and a lumbar bone density decreased more significantly. Compared with the model group, those parameters were significantly higher in high dose group of ginsenosides rats (P<0.05).Conclusion:Ginsenoside can prevent and treat osteoporosis by improving biochemical markers of bone metabolism and bone biomechanics and antagonizing bone loss in glucocorticoid reduce osteoporosis.