To explore mechanism of action of emotional stimulation on onset of TypeⅡ diabetes, i.e. qi disorder syndrome; and the regulatory action of Xinshen NO.5, a compound prescription with effect of regulating qi, countering abnormal ascending qi and dispersing accumulation.Methods:The DM disease was induced in rats by abdominal injecting of small dosage of STZ on top of psycho physiological stimulation (rotation, restraint and crowding). The subjects were divided to normal group, stress group, STZ group, stimulation plus STZ group and Chinese medical treatment group. The serum epinephrine and norepinephrine levels, and the regulatory action of compound formula Xinshen NO. 5 were observed.Results:Chronic emotional stimulation increased serum epinephrine and norepinephrine levels in STZ rats. The stimulation plus STZ group has the highest epinephrine level (P<0.01), which could be down regulated by the Chinese medicine.Conclusion:Serum epinephrine and norepinephrine increase caused by emotional stimulation is one of the mechanism of typeⅡ diabetes in rats, on which Xinshen NO.5 has significant preventing effect.