To discuss Yin Yang of Chinese medicine from the perspective of Dao De Jing deepens our understanding of not only the book but also the culture of Tao from one hand, from the other hand, of the abstract, profound, mysterious but especially importance concept of yin and yang, and toward Chinese medicine with a long history on the whole.Therefore, it’s a novel, valuable methods suitable for wider use.Yin Yang of Chinese medicine is explained from eight different aspects provided from the perspective of Dao De Jing.