To study the characteristic of infrared thermal images and skin conductance，SpO₂ ， heart rate of different constitution human body and its immediate changes after moxibustion and discuss the mechanism of adjusting constitution with moxibustion. Methods:These physiological indexes were recorded， while moxibustion were being carried on the acupoints of bilateral Laogong， Shenque， bilateral Zusanli and bilateral Yongquan in sequence of 45 subjects who had been divided into 4 constitution groups， and infrared thermal images were acquired both before and after moxibustion.Results: Before moxibustion， the infrared thermal images data of Yang-deficiency subjects’ eyes and thighs were lower than normal subjects， but Yin-deficiency subjects’ were higher than normal subjects’， and the infrared thermal images data of Shenque area and heart rate of abnormal constitution subjects’ were lower than normal subjects’; Yang-deficiency subjects’ skin conductance and Yin-deficiency subjects’ SpO₂ lower than normal subjects’，and Yang-deficiency subjects’ SpO₂ and Yin-deficiency subjects’ skin conductance larger than normal subjects’; Yinyang-deficiency subjects’ skin conductance and SpO₂ were both lower than normal subjects’.But the differences among the 4 constitutions were statistically insignificant(P>0.05). After moxibustion， some infrared thermal images data， heart rate and skin conductance of the 4 constitutions were increased， and the SpO₂ were decreased， and the changes had statistical significance(P<0.05).But the physiological indexes of some subjects changed in the opposite direction， and the proportion was different with different constitutions. Conclusion: The result above indicates the differences of yin， yang， qi and blood and the metabolic activity of different constitutions on one hand， on the other hand， it proves that maybe moxibustion adjust constitution through adjusting temperature， skin conductance， SpO₂ and heart rate.