HPLC法测定抗骨增生片中柚皮苷含量
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Determination of the content of naringin in Kanggu Zengsheng Tablet by HPLC
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    摘要:

    目的:建立抗骨增生片中柚皮苷含量测定方法。方法:采用HPLC法,C18柱色谱柱,流动相乙腈-水-冰醋酸(14∶86∶4),流速1.0mL·min-1,检测波长283nm,柱温40℃。结果:在0.0284~0.0994μg范围内柚皮苷线性关系良好,平均回收率为98.76%,RSD为0.68%。结论:该方法能够对抗骨增生片进行准确、快速的柚皮苷定量检测。

    Abstract:

    The method was established for determination of the content of naringin in Kanggu Zengsheng Tablet. Methods: C18 column was used with gradient elution of Acetonitrile-water-glacial acetic acid (14:86:4) as mobile phase by flow rate of 1.0 mL·min-1. The detection wavelength was 283nm and the column temperature was 40℃. Results: The cabibration curves of naringin was in good linearity over the range of 0.0284~0.0994μg (r=0.9999). The average recoverage of naringin was 98.76% (n=6, RSD 0.68%). Conclusion: The method is accurate and quick for determinating the content of naringin in Kanggu Zengsheng Tablet.

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张智军,崔华,马久太. HPLC法测定抗骨增生片中柚皮苷含量[J].世界中医药,2012,7(4).

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  • 收稿日期:2012-04-11
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  • 在线发布日期: 2012-11-19
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