To establish an HPLC method for determination of Saponins of Notoginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra in Gaoguanping capsules.Methods:The HPLC was performed on Hibar C18(250mm×4.6mm)，acetonitrile as mobile phase A，water as mobile phase B. The flow rate was 1.0 mL·min^-1. The detection wavelength was 203 nm. Gradient elution: 0 ～35 min， A: 19%， B: 81%; 35～85 min，A:19% →35%，B: 81% →65%;85～100min， A:35%→19%， B:65→81%. Results: The linear range of notoginsenoside R₁， ginsenoside Rg₁ ginsenoside Re and ginsenoside Rb₁ was 0.4104～3.2832μg， 2.0884～16.7072μg， 1.222～9.776μg， 2.0072～16.0576μg with good correlation. The average recovery was 98.92%， 98.97%， 99.01%， 99.25%， RSD was1.08%、， 1.06%， 0.85%， 1.15%. Conclusion: The method is simple， accurate and reproduciable. It can be used for quality control of notoginsenoside R₁， ginsenoside Rg₁， ginsenoside Re and ginsenoside Rb₁ in Gaoguanping capsules.