To observe the effect of stasis removing herbs plus RNA-based drugs on TNF- alpha in mice with ulcerative colitis (UC). Methods:A total of 42 mice with UC， female or masculinity were chosen and divided into three groups， control group， stasis removing herbs plus RNA-based drugs group and norfloxacin group. After eight-day observation and treatment， we measured the changes of TNF-α. Results:The stasis removing herbs plus RNA-based drugs group presented obvious therapeutic effect. The level of TNF-α reduces to the normal level (P<0.01). Conclusion:Stasis removing herbs plus RNA-based drugs have obvious therapeutic effect on UC， and one of its mechanisms is to reduce the level of TNF-α.