To explore the bidirectional regulation effect of acupuncture and its mechanism.Methods:Rats were administrated via external jugular vein to simulate autonomic nervous activity, which can lead to different conditions of jejunum. Animals were divided into normal control group, propranolol (β2 receptor antagonist) group, and Clenbuterol (β2 receptor agonist) groups, 15 rats in each group. Electro acupuncture（3mA, 2/15Hz）was applied at acupoints of tianshu (ST25), quchi (LI11), shangjuxu (ST37) and dachangshu (BL25) in each group. The intestinal pressure was recorded with a physiological signal acquisition system (RM6240).Results:Unilateral dominance effect was observed in the acupoints of tianshu (ST25), quchi(LI11) and shangjuxu(ST37), and the effect changed according to status of the body; while dachangshu(BL25) showed a bidirectional regulation effect.Conclusion:This indicates that, besides the location of acupoints, the status of the body has a close relationship with the bidirectional regulation effect of acupoint.