To observe the effects of single points as LI11, ST37, ST25, BL25 and agonism and antagonism of their adjunct points on the motilities of jejunum and the diatal colon in anesthetized rats.Methods:In 20 SD（Sprague Dawley）rats anesthetized with urethane (1～1.2g/kg), the intestinal contractility were monitored with manometric balloons put into the jejunum and distal colonic lumens. Acupuncture was randomly performed at four single points as hetertopic points or homotopic points LI11(Quchi), ST37(Shangjuxu), ST25(Tianshu) and BL25(Dachangshu), and six adjunct points as LII+ST37, ST25+BL25, IL11+BL25, IL11+ST25, BL25+ST37, ST25+ST37. Acupuncture manipulation was performed by rotating needles clockwise and anti clockwise at 2 Hz for 1 min.Results:1) Effects of single points on jejunum motility: Acupuncture at either IL11 or ST37 significantly increased the amplitude of jejunum waveform （P＝0011，P＝0003）, and the acupuncture at ST25 significantly decreased jejunum motility（P＝0000）, whereas BL25 had no significant effect on the amplitude of jejunum. Effects of adjunct points on jejunum motility: Acupuncture at LII+ST37 increased amplitude of jejunum（P＝0009）, but there was no obvious agonism effect compared to single points; ST25+LI11, ST25+ST37, ST25+BL25 significantly depressed peristaltic wave amplitudes（P＝0000～0001）, similar to the effect of ST25 single point, indicating that ST25 had dominant effect for adjunct points. 2) Effects of single points on the distal colon motility: Acupuncture at IL11 ST37, ST25 or BL25 accelerated diatal colonic frequencies and amplitudes compared with pre treatment baseline, respectively（P＝0000～0039）. Effects of adjunct points on the diatal colon motility: Acupuncture at IL11+ST37, LI11+ST25 respectively increased the frequencies and amplitudes of the distal colon peristaltic wave（P＝0050,0000）, BL25+ST37, IL11+BL25 increased the amplitude（P＝0002，007）s. And IL11+ST37 showed agonism effect. Conclusion: Findings of the present study indicate that acupuncture at heterotopic points IL11, ST37 produced excitatory effect and at homotopic points ST25, BL25 produced inhibitory effect on jejunum motility respectively, acupuncture at homotopic points IL11, ST37,ST25, BL25 produced excitatory effect on the distal colon motility respectively. The effects of adjunct points are not simply equal to overlying of single points. All adjunct points had neither agonism nor antagonism effect on jejunum, whereas for distal colon, heterotopic adjunct points (LI11+ST37) evoked agonism effect to increase amplitude of motility, and adjunct points with BL25 had antagonism effect to decease frequency and adjunct points (except IL11) with ST25 had an antagonism effect to decrease amplitude